20.500.12556/RUL-153431 Microalgae production on biogas digestate in Sub-Alpine region of Europe—development of simple management decision support tool In a one-and-a-half-year study conducted in the ALS6 region in Europe (Ljubljana, Slovenia), the cultivation of microalgae in anaerobic digestate from food waste, mainly Scenedesmus dimorphus and Scenedesmus quadricauda, was investigated in three ponds (1260 L each) under a greenhouse. The effects of changing digestate quality and quantity as well as seasonal fluctuations on the productivity of the microalgae were investigated in three stages: Learning/Design (SI), Testing (SII), and Verification/Calibration (SIII). A decision support tool (DST) was developed using easy-to-measure parameters such as pH, temperature, electrical conductivity, mineral nitrogen forms and physical, biological parameters (OD, delayed fluorescence intensity). To control optimal pond operation, we proposed the photosynthetic culture index (PCI) as an early indicator for necessary interventions. Flocculation and nitrite levels (above 3 mg NO$_{2-}N$L$^{−1}$) were signals for the immediate remediation of the algae culture. Under optimal conditions in summer SIII, an average algal biomass production of 11 ± 1.5 g m$^{−2}$ day$^{−1}$ and a nitrogen use efficiency of 28 ± 2.6 g biomass/g N-input were achieved with the developed DST. The developed DST tool was, in this study, successfully implemented and used for the cultivation of microalgae consortia predominated by Scenedesmus dimorphus and S. quadricauda with biogas digestate. DST offers the possibility to be modified according to producers’ specific needs, facility, digestate and climate conditions, and as such, could be used for different microalgae cultivation processes with biogas digestate as a food source. circular economy anaerobic digestion food waste digestate microalgae Scenedesmus open ponds decision support tool krožno gospodarstvo anaerobna presnova digestat živilskih odpadkov živilski odpadki mikroalge Scenedesmus odprti ribniki orodja za podporo odločanju true false true Angleški jezik Slovenski jezik Članek v reviji 2024-01-05 08:26:35 2024-01-05 08:26:40 2024-04-24 09:55:38 0000-00-00 00:00:00 2023 0 0 25 str. iss. 24 (16948) Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 2071-1050 179545603 sustainability-15-16948-v2.pdf sustainability-15-16948-v2.pdf 1 AF26BEAD486D0BF3CBE030C2F46BFEF0 97c8e488178a8af19bf10e84fb527bccc8bf81f8efe011e7d799fddccf23be77 1de0596a-ab9c-11ee-8df5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179422 https://www.mdpi.com/2071-1050/15/24/16948 1 c48d50cc-ab9b-11ee-8df5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179421 Biotehniška fakulteta 0 0 0