20.500.12556/RUL-153187 The sensitivity of fungi colonising buckwheat grains to cold plasma is species specific Fungi are the leading cause of plant diseases worldwide and are responsible for enormous agricultural and industrial losses on a global scale. Cold plasma (CP) is a potential tool for eliminating or inactivating fungal contaminants from biological material such as seeds and grains. This study used a low-pressure radiofrequency CP system with oxygen as the feed gas to test the decontamination efficacy of different genera and species commonly colonising buckwheat grains. Two widely accepted methods for evaluating fungal decontamination after CP treatment of seeds were compared: direct cultivation technique or contamination rate method (%) and indirect cultivation or colony-forming units (CFU) method. For most of the tested fungal taxa, an efficient decrease in contamination levels with increasing CP treatment time was observed. Fusarium graminearum was the most susceptible to CP treatment, while Fusarium fujikuroi seems to be the most resistant. The observed doses of oxygen atoms needed for 1-log reduction range from 10$^{24}$–10$^{25}$ m$^{−2}$. Although there was some minor discrepancy between the results obtained from both tested methods (especially in the case of Fusarium spp.), the trends were similar. The results indicate that the main factors affecting decontamination efficiency are spore shape, size, and colouration. Alternaria Aspergillus Cladosporium Epicoccum Fusarium non-thermal plasma decontamination disinfection crops hladna plazma dekontaminacija dezinfekcija poljščine true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-20 15:38:01 2023-12-20 15:38:02 2024-02-27 03:42:07 0000-00-00 00:00:00 2023 0 0 20 str. iss. 6, art. 609 Vol. 9 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57 2309-608X 10.3390/jof9060609 153504259 jof-09-00609-v2.pdf jof-09-00609-v2.pdf 1 015529331C25A7D17C5EE526515C7439 30580995cd21b134118ed91b588e143c6d67bb2ba3d55308f221615bda46f1fd 25ef9cd9-d4ab-11ee-b5a4-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181277 https://www.mdpi.com/2309-608X/9/6/609 1 61521037-9f45-11ee-8a8e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=179114 Biotehniška fakulteta 0 0 0