20.500.12556/RUL-153015 The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ~50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1–2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations. cancer cells magneto-mechanical actuation hexaferrite nanoplatelets cancer cell viability colloidal stability rakotvorne celice true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-14 11:07:19 2023-12-14 11:07:20 2026-05-26 12:06:19 0000-00-00 00:00:00 2024 0 0 Str. 778-787 Vol. 657 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577:537 1095-7103 10.1016/j.jcis.2023.12.019 176772867 1-s2.0-S0021979723023391-main.pdf 1-s2.0-S0021979723023391-main.pdf 1 FC1F2820CDB34713007D93B21ACB2E5B b5d1a210cc2fe40ee0330e8ebd3a8b1080b2c0d65f191c6312960bc2835dc348 e669ffe6-9a68-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178796 https://www.sciencedirect.com/science/article/pii/S0021979723023391 0 925de866-9a68-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178795 Medicinska fakulteta 0 0 0