20.500.12556/RUL-153008 Surfactin molecules with a cone-like structure promote the formation of membrane domains with negative spontaneous curvature and induce membrane invaginations Surfactin uniquely influences lipid bilayer structure by initially inducing membrane invaginations before solubilization. In this study, we exposed DOPC giant vesicles to various surfactin concentrations at different temperatures and observed surfactin-induced membrane invaginations by using differential interference contrast and confocal laser fluorescence microscopy. These invaginations were stable at room temperature but not at higher temperatures. Surfactin molecules induce membrane nanodomains with negative spontaneous curvature and membrane invaginations despite their intrinsic conical shape and intrinsic positive curvature. Considering the experimentally observed capacity of surfactin to fluidize lipid acyl chains and induce partial dehydration of lipid headgroups, we propose that the resulting surfactin-lipid complexes exhibit a net negative spontaneous curvature. We further conducted 3D numerical Monte Carlo (MC) simulations to investigate the behaviour of vesicles containing negative curvature nanodomains within their membrane at varying temperatures. MC simulations demonstrated strong agreement with experimental results, revealing that invaginations are preferentially formed at low temperatures, while being less pronounced at elevated temperatures. Our findings go beyond the expectations of the Israelachvili molecular shape and packing concepts analysis. These concepts do not take into account the influence of specific interactions between neighboring molecules on the inherent shapes of molecules and their arrangement within curved membrane nanodomains. Our work contributes to a more comprehensive understanding of the complex factors governing vesicle morphology and membrane organization and provides insight into the role of detergent-lipid interactions in modulating vesicle morphology. surfactin lipid membranes solubilization dynamics curved membrane nanodomains molecular shape lipidne membrane surfaktin true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-14 09:35:59 2023-12-14 09:36:04 2024-04-03 12:57:11 0000-00-00 00:00:00 2023 0 0 Str. 1193-1200 pt. B Vol. 650 Nov. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.352 0021-9797 10.1016/j.jcis.2023.07.057 159013379 1-s2.0-S0021979723013061-main.pdf 1-s2.0-S0021979723013061-main.pdf 1 0ABA55CEA5E8A99703A28E12BF0B4659 4292145c2d8a664479aadae43847a1a6a1d86ca4d175c223b2832fa17df374b8 30a5a541-9a5c-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178786 https://www.sciencedirect.com/science/article/pii/S0021979723013061 0 d1ea9dc8-9a5b-11ee-91f8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178785 Biotehniška fakulteta Fakulteta za elektrotehniko Medicinska fakulteta Zdravstvena fakulteta 0 0 0