20.500.12556/RUL-152845 Effect of electrochemotherapy on myogenesis of mouse C2C12 cells in vitro Electrochemotherapy (ECT) is a local ablative therapy for the treatment of different skin and subcutaneous tumors and certain tumors in internal organs. Skeletal muscle represents a major tumor-surrounding tissue, exposed to side effects of ECT. At the cellular level, side-effects of ECT on skeletal muscle and underlying mechanisms have not been examined yet. Thus, we aimed to determine the effect of ECT in the mouse muscle cell line C2C12 during in vitro myogenesis. We evaluated the electroporation efficiency and viability of C2C12 myotubes at increasing voltages (200–1300 V/cm) using propidium iodide (PI). Permeabilization of PI into the cells was voltage-dependent accounting up to 97 % efficiency at the highest voltage. High cell viability and myotube integrity were maintained until 4 days after electroporation. ECT with the cytostatic drugs bleomycin and cisplatin decreased the viability of C2C12 myoblasts and myotubes in a dose-dependent manner. However, myoblasts were more sensitive to ECT than myotubes. Increased secretion of IL-6, observed 3 days after ECT, confirming its effects on early myogenesis. Only minor effects of ECT were observed in treated myotubes. These results contribute to the safety profile of ECT in tumor treatment. electroporation electrochemotherapy myogenesis bleomycin cisplatin skeletal muscle elektroporacija elektrokemoterapija miogeneza true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-08 15:46:05 2023-12-08 15:46:09 2023-12-09 03:49:42 0000-00-00 00:00:00 2023 0 0 7 str. art. 108487 Vol. 153 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-083 1567-5394 10.1016/j.bioelechem.2023.108487 156727811 1-s2.0-S156753942300124X-main.pdf 1-s2.0-S156753942300124X-main.pdf 1 2BB1226260E890A5B3B5C3BE5F709E4B 6e95067c154d02cf7020c82fee32aaa48ce5b91c097b3534119bec65577d0275 1dba6867-95d9-11ee-9c20-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178538 https://www.sciencedirect.com/science/article/pii/S156753942300124X 1 868c8efe-95d8-11ee-9c20-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178536 Medicinska fakulteta 0 0 0