20.500.12556/RUL-152740 Simple HPLC-UV method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1–10 mg/L for oxcarbazepine; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy. antiepileptic drugs human plasma therapeutic drug monitoring bioanalytical method validation high-performance liquid chromatography antiepileptična zdravila človeška plazma terapevtsko spremljanje zdravil validacija bioanalitskih metod tekočinska kromatografija visoke ločljivosti epilepsija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-05 12:02:42 2023-12-05 12:02:47 2023-12-06 03:40:31 0000-00-00 00:00:00 2023 0 0 15 str. iss. 23, art. 7830 Vol. 28 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544.5:616.853 1420-3049 10.3390/molecules28237830 174900739 molecules-28-07830-v2.pdf molecules-28-07830-v2.pdf 1 6F72B0E91D003E7D14C18E4A7AABE89A f9e957c6939281b153cfb0c1ad4f612e1ded5229a3dece49f393b8324591778f a5afde72-935e-11ee-867f-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178359 https://www.mdpi.com/1420-3049/28/23/7830 1 d1d2878c-935d-11ee-867f-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178358 Fakulteta za farmacijo 0 0 0