20.500.12556/RUL-152679 Comparison of 3 enzyme-linked immunoassay methods to evaluate serum concentrations of infliximab and antibodies to infliximab in 32 patients with moderate to severe inflammatory bowel disease BACKGROUND: Monitoring of trough levels and anti-drug antibodies is important when patients with inflammatory bowel disease (IBD) are treated with anti-TNF biologics due to guided therapeutic decisions. The comparability of 3 ELISA tests for detection of the lowest serum concentration of infliximab (IFX) or antibodies to IFX (ATIs) was evaluated. MATERIAL AND METHODS: Two commercial assays for measuring IFX levels were compared with the in-house (UHL) test. ATIs were measured with 1 commercial test and compared to the in-house test. According to the guidelines, IFX levels were within the range of 3 to 7 µg/mL. RESULTS: The decision to continue therapy would be the same for 11 out of 16 patients when comparing the apDia Infliximab ELISA and UHL test, and for 12 out of 18 patients when comparing the Lisa-Tracker and in-house UHL test. Linear correlations between the tests were R=0.92 (UHL and apDia), R=0.91 (apDia and Lisa-Tracker), and R=0.89 (UHL and Lisa-Tracker) with P<0.001, respectively. CONCLUSIONS: As the IFX levels are important for decisions on further therapy, detectable IFX levels realistically reflect the presence of the drug in the patients’ blood and thus control inflammatory activity. The tests were found as comparable and performed well in this aspect and might be used in everyday clinical practice. antibodies enzyme-linked immunosorbent assay infliximab materials testing protitelesa encimski imunski test infliksimab testiranje materialov true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-04 11:27:56 2023-12-04 11:27:58 2023-12-05 03:41:06 0000-00-00 00:00:00 2023 0 0 10 str. art. e939084 Vol. 29 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2023-01-01 615.32:616.34 1643-3750 10.12659/MSM.939084 140979971 https://medscimonit.com/abstract/index/idArt/939084 1 cbb64f4a-928f-11ee-9ca9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178284 939084.pdf 939084.pdf 1 5E82C3A30CCE85FC7CDC514E46E630FA 3a9a03854919d7f1e23489e9cb44ccc43c5d3328d078a324ebc452f087f852d7 5aa68a49-9290-11ee-9ca9-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178285 Zdravstvena fakulteta Medicinska fakulteta 0 0 0