20.500.12556/RUL-152632 Fallopia japonica and Fallopia $\times$ bohemica extracts cause ultrastructural and biochemical changes in root tips of radish seedlings Japanese knotweed (Fallopia japonica) and Bohemian knotweed (Fallopia $\times$ bohemica) are invasive plants that use allelopathy as an additional mechanism for colonization of the new habitat. Allelochemicals affect the growth of roots of neighboring plants. In the present study, we analyze the early changes associated with the inhibited root growth of radish seedlings exposed to aqueous extracts of knotweed rhizomes for 3 days. Here, we show that cells in the root cap treated with the knotweed extracts exhibited reduced cell length and displayed several ultrastructural changes, including the increased abundance of dilated ER cisternae filled with electron-dense material (ER bodies) and the accumulation of dense inclusions. Moreover, mitochondrial damage was exhibited in the root cap and the meristem zone compared to the non-treated radish seedlings. Furthermore, malfunction of the intracellular redox balance system was detected as the increased total antioxidative capacity. We also detected increased metacaspase-like proteolytic activities and, in the case of 10% extract of F. japonica, increased caspase-like proteolytic activities. These ultrastructural and biochemical effects could be the reason for the more than 60% shorter root length of treated radish seedlings compared to controls. allelochemicals proteases programmed cell death alelokemikalije proteaze programirana celična smrt true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-01 11:08:04 2023-12-01 11:08:05 2024-10-02 13:14:49 0000-00-00 00:00:00 2023 0 0 15 str. iss. 5, art. e14032 Vol. 175 Sep./Oct. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 581.1:577.15 0031-9317 10.1111/ppl.14032 166181123 Physiologia_Plantarum_-_2023_-_oln_-_Fallopia_japonica_and_Fallopia___bohemica_extracts_cause_ultrastructural_and.pdf Physiologia_Plantarum_-_2023_-_oln_-_Fallopia_japonica_and_Fallopia___bohemica_extracts_cause_ultrastructural_and.pdf 1 D802C9C9624DF6C393EF88CECBC06BCD 36993b094b81a6c58a829b0fcdfa5beadbc9160b950cce38d43285ae1551f0d0 d9f61d23-9031-11ee-b3b7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178216 https://onlinelibrary.wiley.com/doi/10.1111/ppl.14032 1 8553a27f-9031-11ee-b3b7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178215 Biotehniška fakulteta Fakulteta za kemijo in kemijsko tehnologijo 0 0 0