20.500.12556/RUL-152544 Cold atmospheric plasma for surface disinfection a promising weapon against deleterious meticillin-resistant Staphylococcus aureus biofilms Background: Bacteria are becoming increasingly resistant to classical antimicrobial agents, so new approaches need to be explored. Aim: To assess the potential of cold atmospheric plasma for the management of methicillin-resistant Staphylococcus aureus (MRSA). Methods: The 24, 48, and 72 h resistant and susceptible S. aureus biofilms were exposed to 60, 120, and 180 s treatment with plasma. Findings: Increasing the treatment time results in higher cell reduction for both susceptible and resistant strains of S. aureus (P < 0.05). Up to log$_{10}$ reduction factor of 5.24 cfu/cm$^2$ can be achieved in 180 s of plasma treatment. Furthermore, plasma can substantially alter the cell’s metabolisms and impact cell membrane integrity. However, it has not been shown that plasma can reduce biofilm biomass in the case of 24 h and 48 h biofilms, although the 72 h biofilm was more susceptible, and its biomass was decreased (P < 0.05). The accumulation of intrabacterial reactive oxygen species was also observed, which confirms the plasma’s induction of oxidative stress. Finally, it was shown that continuous plasma exposure of bacterial cells does not cause resistance to plasma, nor is resistance developed to cefoxitin. Conclusion: Cold atmospheric plasma is a good candidate for S. aureus and MRSA biofilm treatment and may therefore be of value in the bacterial resistance crisis. cold atmospheric plasma Staphylococcus aureus MRSA reduction metabolic activity resistance hladna atmosferska plazma Staphylococcus aureus MRSA zmanjšanje presnovna aktivnost odpornost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-27 11:37:33 2023-11-27 11:37:34 2023-12-21 14:21:58 0000-00-00 00:00:00 2024 0 0 Str. 64-75 Vol. 143 Jan. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616.9:614 0195-6701 10.1016/j.jhin.2023.10.014 173756675 Cold_atmospheric_plasma_for_surface_disinfection.pdf Cold_atmospheric_plasma_for_surface_disinfection.pdf 1 7471C74B06EB1BDD5A40431FE0CA62C1 6a852043732fe18fc665d4cfbbebff350591fc1a7c7099cb81172c6bdd5f4f7a 67fdc62f-8d11-11ee-8ac8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178072 https://www.sciencedirect.com/science/article/pii/S0195670123003493 1 fa259943-8d10-11ee-8ac8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178071 Zdravstvena fakulteta Biotehniška fakulteta 0 0 0