20.500.12556/RUL-152407 Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates and delays senescence Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the stress is no longer present. Persistence of SGs due to mutations or chronic stress has been often related to age-dependent protein-misfolding diseases in animals. Here, we find that the metacaspase MC1 is dynamically recruited into SGs upon proteotoxic stress in Arabidopsis (Arabidopsis thaliana). Two predicted disordered regions, the prodomain and the 360 loop, mediate MC1 recruitment to and release from SGs. Importantly, we show that MC1 has the capacity to clear toxic protein aggregates in vivo and in vitro, acting as a disaggregase. Finally, we demonstrate that overexpressing MC1 delays senescence and this phenotype is dependent on the presence of the 360 loop and an intact catalytic domain. Together, our data indicate that MC1 regulates senescence through its recruitment into SGs and this function could potentially be linked to its remarkable protein aggregate-clearing activity. stress granules biomolecular condensates protein aggregates metacaspases heat stress senescence stresne granule biomolekularni kondenzati proteinski agregati metakaspaze toplotni stres staranje true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-23 12:58:43 2023-11-23 12:58:44 2023-11-24 03:54:53 0000-00-00 00:00:00 2023 0 0 Str. 3325–3344 iss. 9 Vol. 35 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.15 1040-4651 10.1093/plcell/koad172 157990915 koad172.pdf koad172.pdf 1 9076AB8FDE54DA465F0E9B9B013379FE 639dde0820645e196bfd314f2d5d560100bb8bda787c63df282318e5308e6cdd f1714122-89f7-11ee-a702-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177910 https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koad172/7208185 1 b0658d0c-89f7-11ee-a702-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177909 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0