20.500.12556/RUL-152346 Sex-dependent differences in blood-urine barrier are subtle but significant in healthy and chronically inflamed mouse bladders The urothelium is a vital permeability barrier that prevents the uncontrolled flow of urinary components into and out of the bladder interstitium. Our study addressed the question of possible sex-specific variations in the urothelium of healthy mice and their impact on chronic bladder inflammation. We found that healthy female bladders have a less robust barrier function than male bladders, as indicated by significant differences in transepithelial electrical resistance (TEER) values. These differences could be attributed to detected higher claudin 2 mRNA expression and a less pronounced glycocalyx in females than in males. In addition, TEER measurements showed delayed barrier recovery in chronically inflamed female bladders. We found subtle differences in the expressions of genes involved in the regulation of the actin cytoskeleton between the sexes, as well as pronounced urothelial hyperplasia in females compensating for attenuated barrier function. The identified genetic variations in glycosylation pathways may also contribute to this divergence. Our findings add to the growing body of literature on the intricate sex-specific nuances of urothelial permeability function and their implications for chronic bladder inflammation. Understanding these differences could lead to tailored diagnostic and therapeutic approaches in the treatment of bladder disorders in the future. bladder blood–urine barrier chronic cystitis sex differences mehur krvno-urinska pregrada kronični cistitis spolne razlike sečni mehur urotelij true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-21 12:54:10 2023-11-21 12:54:14 2024-12-10 14:20:00 0000-00-00 00:00:00 2023 0 0 19 str. iss. 22, art. 16296 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 611.6.018.7 1422-0067 10.3390/ijms242216296 172994563 ijms-24-16296.pdf ijms-24-16296.pdf 1 867E1753699B5E2C7C49A0C1E3C62754 b98517e04c4ef41f641040f10d519e0787ddc3205947046157f54fe1b1dc4911 2f5df0bf-8865-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177811 https://www.mdpi.com/1422-0067/24/22/16296 1 b1939cfd-8864-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177810 Medicinska fakulteta Fakulteta za farmacijo Veterinarska fakulteta 0 0 0