20.500.12556/RUL-152336 Leptospirosis and characterization of Leptospira isolates from patients in Koprivnica-Križevci County, Croatia from 2000–2004 Introduction. Leptospirosis, caused by spirochetes of the genus Leptospira, is present in the Koprivnica-Križevci County area, Croatia. Clinical manifestation can range from asymptomatic, short-term mild, non-specific febrile disease, to severe forms with high mortality rates. Aim. The aim of the study was to valuate culture in front of microscopic agglutination test (MAT) for diagnosis of infection, and to evaluate clinical and laboratory features of the disease. Moreover, we want to characterize Leptospira strains involved in infection in Koprivnica-Križevci County, Croatia. Methods. We included 68patients with clinical presentation consistent with leptospirosis collected in a 5-year period (2000–2004). Clinical samples (blood, urine and cerebrospinal fluid, CSF) were inoculated in Kolthoff’s medium; species of isolated Leptospira strains was determined with Tm of real-time PCR, serogroup/serovar with MAT and NotI-RFLP analysis. Demonstration of specific antibodies in patients’ sera was done using microscopic agglutination test. Results. Leptospira was isolated from the blood of 14/51(27.5%) patients and the most often identified serogroup/serovar was Icterohaemorrhagiae (8/10, 80%) followed by Grippotyphosa (10%). Regarding to species level, 8/10 isolated belonged to L. interrogans sensu stricto and one to L. kirschneri species. MAT was carried out on 51patients with suspected leptospirosis, and was positive in 11/51(21.5%) patients. Most of our patients presented with moderate severe symptoms, were hospitalized from August to October, and were infected mainly during the work or recreation in our county. The frequency of particular clinical features and pathological laboratory findings correlated with the severity of the clinical condition. Conclusions. Leptospirosis can be confirmed microbiologically, culture and MAT contributed almost equally to the diagnosis of infection. Serovar Icterohaemorrhagiae was found as the dominant one, and L. interrogans sensu stricto as dominant species in our county. Epidemiological data shown that leptospirosis occurs seasonally, affects the rural population, and most commonly is presented with moderate severe clinical course. leptospirosis Leptospira isolation Leptospira species Leptospira serogroup Leptospira serovar MAT Koprivnica-Križevci County Croatia MLST sequence type leptospiroza Leptospira izolati Hrvaška zaporedje vrste mikrobiologija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-21 08:44:47 2023-11-21 08:44:56 2023-11-22 03:38:21 0000-00-00 00:00:00 2023 0 0 12 str. iss. 4, art. 000431 Vol. 5 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9:579 2516-8290 10.1099/acmi.0.000431 168212995 acmi000431.pdf acmi000431.pdf 1 AEB0E67FCB4C0B3B3EE25DA9934827D8 69193d9bcf5830f50cd5fcfef0f02ee7507f8a0f479a06bdda5cfde09a34677c 37593265-8842-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177794 https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000431 1 de8b7f20-8841-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177792 Medicinska fakulteta 0 0 0