20.500.12556/RUL-152335 High throughput laser ablation ICP-MS bioimaging of silver distribution in animal organisms and plant tissue after exposure to silver sulfide nanoparticles Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a method with high potential to visualize the distribution of elements in different samples, including a variety of organisms. This study aimed to demonstrate the broad application of LA-ICP-MS in nanomaterial-biota fate studies as a high throughput bioimaging tool and to broaden the choice of standard organism models in material-biota interaction research. In this study, operation parameters of LA-ICP-MS were optimised on one organism, woodlice Porcellio scaber. Subsequently, the scanning conditions were tested on a range of aquatic (Girardia tigrina, Lumbriculus variegatus, and Oncorhynchus mykiss), terrestrial organisms (Lumbricus rubellus, Porcellio scaber) and one plant (Triticum aestivum) upon exposure to Ag$_2$S nanoparticles (NPs) and silver nitrate (AgNO$_3$). Model organisms were exposed in aquatic or terrestrial mesocosm experiments where nominal concentrations of Ag were 10 μg Ag per L of water and 10 mg Ag per kg of soil, respectively. The results showed that both sample preparation and LA-ICP-MS imaging conditions, as optimized on the selected organism (65 μm laser diameter, scan rate 100 μm s$^{−1}$, measuring duration 35 min), are applicable on different tissues. These LA-ICP-MS imaging conditions enable recognition of the main biological structures and biodistribution of elements of interest. By using fast-screening LA-ICP-MS, we confirmed the presence of Ag$_2$S NPs on the body surface or in the gut lumen (adsorbed and retained), but not in other internal parts of organisms, which is consistent with our previous toxicokinetic studies. The presence of Ag was also confirmed in some parts of wheat roots. The advantage of this technique is the possibility of sequential use of fast- and slow-scanning steps to optimise the duration of analysis and data processing, whilst also improving cost-effectiveness without compromising the quality of results. laser ablation ICP-MS bioimaging animal organisms plant tissue silver sulfide nanoparticles laserska ablacija ICP-MS biološko slikanje živalski organizmi rastlinska tkiva nanodelci srebrovega sulfida true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-21 08:33:32 2023-11-21 08:33:34 2023-11-22 03:38:21 0000-00-00 00:00:00 2023 0 0 Str. 2396-2404 iss. 11 Vol. 38 1 Nov. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-11-01 543.51:620.3 0267-9477 10.1039/D3JA00223C 172868611 RAZ_Marolt_Gregor_2023.pdf RAZ_Marolt_Gregor_2023.pdf 1 8F0C038D58BA3D0307722C507BC8176E 639d1e7a122712959547ab5adc761eed218b9b7a6b7b12160ed9d2b7a849c27e d848d789-8841-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177790 https://pubs.rsc.org/en/content/articlelanding/2023/ja/d3ja00223c 1 48185191-8840-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177789 Fakulteta za kemijo in kemijsko tehnologijo Biotehniška fakulteta 0 0 0