20.500.12556/RUL-152230 Genomic insights into the safety and bacteriocinogenic potential of isolates from artisanal fermented milk Matsoni Matsoni is a fermented milk common in the Caucasian region. In this study, whole-genome sequencing was performed with the aim of determining the taxonomic position of 25 previously selected candidate Matsoni starter cultures, identifying bacteriocin genes and prophages in their genomes, and evaluating their safety. In silico prediction of antibiotic resistance genes, virulence factors, bacteriocin genes and prophages was performed using dedicated databases and bioinformatics tools. With the exception of Enterococcus faecalis, which contained the mobile genetic element Tn916 with tetM and virulence factors, and Streptococcus thermophilus with mefE, the isolates were found to be safe for use as starter cultures. Thermophilin A, thermophilin 13, streptide and Blp genes were predicted in isolates of Streptococcus thermophilus, and genes for enterocins A, B, and X were found in Enterococcus faecium, but inhibition between strains was not observed. Several complete prophages were also detected in the genomes of Lactobacillus delbrueckii ssp. lactis. Lacticaseibacillus rhamnosus, Limosilactobacillus fermentum, Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium. Some of these isolates are good candidates for customised autochthonous Matsoni starter cultures which are an alternative to the commercial starter cultures currently used in the industry. whole genome sequencing fermented milk prophages bacteriocins tetM mefE mlečni izdelki fermentirano mleko matsoni genetika genom sekvenciranje bakteriocini profagi true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-13 10:52:56 2023-11-13 10:52:57 2023-12-21 13:43:08 0000-00-00 00:00:00 2023 0 0 13 str. art. 115183 Vol. 185 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 637.1:575 1096-1127 10.1016/j.lwt.2023.115183 162265603 1-s2.0-S0023643823007624-main.pdf 1-s2.0-S0023643823007624-main.pdf 1 D07C4B027D40D49AEDAB939A79BAC2F8 831ebe79114b53d5376267dc8e5bca70e7e5a53bec55257d823fca97fbbe89bb d3d7e940-820b-11ee-a249-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177594 https://www.sciencedirect.com/science/article/pii/S0023643823007624 0 6d26d663-820a-11ee-a249-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177593 Biotehniška fakulteta 0 0 0