20.500.12556/RUL-152053 Vsebnost in sestava eteričnega olja različnih populacij navadne dobre misli (Origanum vulgare L.) Essential oil content and composition in different populations of oregano (Origanum vulgare L.) Navadna dobra misel spada v družino ustnatic (Lamiaceae). Je zelnata trajnica, poznana predvsem kot aromatična dišavnica, ki pa je tudi zdravilna rastlina. Z diplomskim delom smo želeli na 5 populacijah navadne dobre misli (O. vulgare ssp. vulgare), prinesenih iz različnih naravnih rastišč v Sloveniji in razmnoženih na dveh lokacijah Laboratorijskega polja Biotehniške fakultete v Ljubljani (na Laboratorijskem polju BF v sklopu nasada Javna služba rastlinske genske banke (JSRGB) in na Laboratorijskem polju BF izven nasada JSRGB) ter vzorcu dlakave podvrste navadne dobre misli (grški origano) (O. vulgare ssp. hirtum), shranjenega iz leta 2016, določiti vsebnost eteričnega olja (EO) z metodo vodne destilacije ter z metodo plinske kromatografije (GC) analizirati njihovo sestavo. Izmerili smo volumen EO za vsako od populacij in ga izrazili v ml/kg rastlinske droge. Povprečne vsebnosti EO izmerjene v ml so se gibale med 0,038 ml (populacija M1) in 0,2 ml (populacija M1BT). Najnižjo povprečno vsebnost EO izraženo v ml/kg je imela populacija M1 (1,27 ml/kg), najvišjo pa vzorec 9/1 iz leta 2016 (15,00 ml/kg). Analiza sestave EO in aromatične vode (AV) je pri večini populacij podvrste O. vulgare ssp. vulgare pokazala preplet cimil in sabinil kemotipa s prisotnostjo seskviterpenov, medtem ko je vzorec grškega origana (O. vulgare ssp. hirtum) imel cimil kemotip, saj je EO vsebovalo predvsem karvakrol (61,8 %). Karvakrol se je v manjšem odstotku pojavil tudi pri EO populacije M4 (I) (6,07 %), pri AV populacije M4 (II) (15,43 %) in EO populacije M4 (II) (3,76 %), ter EO populacije M1 (1%). V analiziranih vzorcih je bila možna prisotnost timola le v AV populacije 9/1. The common oregano belongs to the mint family (Lamiaceae). It is a herbaceous perennial mainly known as an aromatic herb, however it is also a medicinal plant. In our graduation thesis we wanted to use 5 populations of O. vulgare ssp. vulgare, brought from different natural habitats in Slovenia and propagated at two locations of the Laboratory Field of the Biotechnical Faculty in Ljubljana (at the BF Laboratory Field within the Plantation of the Public Service of the Plant Gene Bank (PSPGB) and at the BF Laboratory Field outside the PSPGB plantation) and a sample of 1 population of O. vulgare ssp. hirtum, saved from 2016, to determine the content of essentiala oil (EO) using the water distillation method and to analyze their composition using the gas chromatography (GC) method. We measured the volume of EO for each of the populations and expressed it in ml/kg of plant drug. Average EO contents measured in ml ranged between 0,038 ml (population M1) and 0,2 ml (population M1BT). Population M1 had the lowest average EO content expressed in ml/kg (1,27 ml/kg), while sample 9/1 saved from 2016 had the highest (15,00 ml/kg). The analysis of the composition of EO and aromatic water (AV) in most populations of the subspecies O. vulgare ssp. vulgare showed a combination of cymyl and sabinyl chemotype with the presence of sesquiterpenes, while Greek oregano (O. vulgare ssp. hirtum) had a cymyl chemotype, as the EO contained mainly carvacrol (61.8 %). Carvacrol also appeared in a smaller percentage in EO of population M4 (I) (6.07 %), in AV of population M4 (II) (15.43 %), in EO of population M4 (II) (3.76 %), and in EO population M1 (1 %). In the analyzed samples, thymol was possibly present only in the aromatic water of population 9/1. navadna dobra misel O. vulgare ssp. vulgare O. vulgare ssp. hirtum zdravilna rastlina eterično olje vodna destilacija plinska kromatografija vsebnost kemotip karvakrol common oregano O. vulgare ssp. vulgare O. vulgare ssp. hirtum medicinal plant essentiala oil water distillation gas chromatography content chemotype carvacrol true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-10-29 07:15:07 2023-10-29 07:15:18 2023-10-30 15:26:32 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 232640 170375939 34479.pdf 34479.pdf 1 3101F9CA1EF61D9A086948CEA145AB74 a053291e17da805b6f2065088c548d36ae5d1f417b716883a5b45573d68c99e2 98a94df8-7622-11ee-956b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177327 Biotehniška fakulteta 0 0 0