20.500.12556/RUL-151975 Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia Enterococci represent a significant part of the non-starter LAB microbiota of artisanal cheeses produced mainly from raw milk. Common approaches to safety evaluation of enterococci isolates include assessment of antimicrobial resistance and virulence potential. Hence, a collection of 47 (n = 22, Serbia; n = 25, Slovenia) dairy enterococcal isolates, of which E. faecalis (n = 28), E. faecium (n = 11), E. durans (n = 5), E. cas- seliflavus (n = 2), and E. gallinarum (n = 1), was analyzed. The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of the selected antimicrobial resistance and virulence genes was investigated using PCR. Isolates were resistant to tetracycline (TET) (25.5%), erythromycin (ERY) (17.0%), gentamycin and chloramphenicol (CHL) (∼6%). No resistance to ampicillin (AMP), ciprofloxa- cin (CIP), daptomycin (DAP), linezolid (LZD), teicoplanin (TEI), tigecycline (TGC) and vancomycin (VAN) was detected. Among all the resistance determinants analyzed, ermB gene was detected most frequently. All 10 virulence genes analyzed were detected with a distribution of cpd (72.3%), cob and ccf (70.2%), gelE (68.1%), hyl (59.6%), agg (53.2%) and esp (46.8%). The genes encoding cytolysin (cylA, cylM and cylB) were amplified to a lesser extent (21.3%, 21.3% and 12.8%, respectively). However, due to the limited number of enterococci isolates analyzed in the present study, further studies are still required in order to better docu- ment the safety status of dairy enterococci. Enterococci white cheese antimicrobial resistance virulence determinants mleko mlečni izdelki avtohtoni siri mikrobiologija enterokoki Slovenija Srbija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-26 12:36:53 2023-10-26 12:36:57 2023-10-27 04:02:18 0000-00-00 00:00:00 2023 0 0 Str. 765-775 iss. 7 Vol. 29 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1532-1738 10.1177/10820132221117870 117510403 dordevic-et-al-2022-safety-evaluation-of-enterococci-isolated-from-raw-milk-and-artisanal-cheeses-made-in-slovenia-and.pdf dordevic-et-al-2022-safety-evaluation-of-enterococci-isolated-from-raw-milk-and-artisanal-cheeses-made-in-slovenia-and.pdf 1 464BAF80FCBBDA79258EC0C17CA9C61B f63cc86c7d19d31aeb1dfed902f736c3b0676ae85882c7409d75c195802fe3bf ad1427f7-73ed-11ee-a653-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177149 https://journals.sagepub.com/doi/10.1177/10820132221117870 1 9710e51b-73eb-11ee-a653-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177148 Veterinarska fakulteta Biotehniška fakulteta 0 0 0