20.500.12556/RUL-151931 Mikrokapsulacija folne in askorbinske kisline v ionotropne hidrogele Microencapsulation of folic and ascorbic acid in ionotropic hydrogels V magistrski nalogi smo preverjali ali je ionotropni gel (iz mešanice alginata in pektina) sposoben učinkovito zadržati askorbinsko (AA) in folno kislino (FA). Cilj naloge je, da pripravimo stabilne mikrokapsule z askorbinsko in folno kislino in pri tem dosežemo visoko kapsulacijsko učinkovitost ter z ustreznim razmerjem kapsul izpolnimo priporočen dnevni vnos za obe komponenti. Spremljali smo kapsulacijsko učinkovitost, sproščanje bioaktivnih komponent v raztopino CaCl2, sproščanje obeh bioaktivnih komponent pri simuliranih gastrointestinalnih pogojih in pogledali mikrokapsule pod mikroskopom. Folno kislino smo uspešno kapsulirali in pri tem dosegli visoko učinkovitost (99 %) med tem, ko smo pri kapsulaciji askorbinske kisline prišli do 25 % učinkovitosti. Askorbinska kislina se je sprostila v časovnem oknu 20 min, folna kislina pa je ostala stabilna več dni. V obeh primerih smo dosegli priporočen dnevni vnos z ustrezno zatehto kapsul. Sproščanje folne kisline je bilo manjše pri kislih pogojih, med tem ko je bilo pri alkalnih pogojih večje. Pri nižjem pH se folna kislina imobilizira in ujame v mrežo nosilnega materiala, pri višjih pH pa je nosilni material (alginat) negativno nabit, kar povzroči nabrekanje in sproščanje FA (folne kisline). Poleg tega je topnost FA prialkalnem pH višja. Sproščanje AA je bilo odvisno od pH, saj opazimo razlike v sproščanju pri različnih pH (2,0, 5,0, 7,0). Najhitreje se je AA sproščala pri pH 7,0, znano je, da AA v prosti raztopini postane manj stabilna, ko se pH približuje nevtralnemu, vendar moramo poudariti, da ni bilo bistvenih razlik, saj je v časovnem okviru 20 min pri vseh treh pH vrednostih prišlo do izgube večine AA. In this thesis, we test whether an ionotropic gel (made of a mixture of alginate and pectin) is able to effectively retain ascorbic and folic acid. The aim of the thesis is to prepare stable microcapsules with ascorbic and folic acid, achieving a high encapsulation efficiency and meeting the recommended daily intake for both components with an appropriate capsule ratio. We monitored the encapsulation efficiency, the release of the bioactive components into the CaCl2 solution, the release of both bioactive components under simulated gastrointestinal conditions and looked at the microcapsules under a microscope. Folic acid was successfully encapsulated, achieving a high efficiency (99%), while ascorbic acid encapsulation reached 25% efficiency. Ascorbic acid was released within a time window of 20 min, while folic acid remained stable for several days. In both cases, the recommended daily intake was achieved with the appropriate capsule weight. The release of folic acid was lower under acidic conditions, while it was higher under alkaline conditions. At lower pH, folic acid is immobilised and trapped in the network of the carrier material, whereas at higher pH the carrier material (alginate) becomes negatively charged, causing swelling and release of FA. In addition, the solubility of FA is higher at alkaline pH. The release of FA was pH dependent, as differences in release were observed at different pH (2,0, 5,0, 7,0). The fastest release of AA was at pH 7,0, it is known that ascorbic acid in free solution becomes less stable as the pH approaches neutral, but it should be noted that there was no significant difference as most of the AA was lost within a time frame of 20 min at all three pH values. kapsulacija askorbinska kislina folna kislina alginat pektin hidrogeli mikrokapsule ekstruzija curka tekočine encapsulation ascorbic acid folic acid alginate pectin hydrogels microcapsules liquid jet extrusion true false false [L. Oluić] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-10-26 07:15:19 2023-10-26 07:15:34 2023-10-27 04:02:11 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.164.1:615.014.6 232600 169943299 38080.pdf 38080.pdf 1 88FECEA2E4582CEE508D637E8A597D7A ab30b5ab50f5a4e4ea55ea2ed5e2d8108ea208f7e506d5f33b81f42cdd73dab1 bcaadb20-73be-11ee-a653-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177087 Biotehniška fakulteta 0 0 0