20.500.12556/RUL-151814 Model-based design of continuous biotransformation in a microscale bioreactor with yeast cells immobilized in a hydrogel film research data underlying the article Miniaturized flow reactors with immobilized biocatalysts offer enormous potential for process intensification. They enable long-term use of biocatalysts, continuous operation that significantly exceeds the performance of batch processes, and efficient mass and heat transfer leading to highly controlled reaction conditions. Despite their increasing use in biocatalytic processes, optimization of reactor design and operating conditions based on a mathematical description is very rare. This work aims to fill this gap by developing and validating a mathematical model for the continuous biotransformation process in a microreactor between two plates with immobilized whole cells in hydrogel layers on the bottom and top of the reactor. A biocatalytic production of L-malic acid by fumaric acid hydration using permeabilized Saccharomyces cerevisiae whole cells was used as a model reaction. The diffusivity of substrate and product in a liquid phase and in a copolymer hydrogel layer, as well as the reaction kinetic parameters considering the Michaelis-Menten kinetics of the reversible enzymatic reaction, were estimated in initial batch experiments. The results obtained in a continuously operated microbioreactor with immobilized whole cells at different fumaric acid concentrations and flow rates were in excellent agreement with the predictions of the developed mathematical model encompassing transport phenomena and reaction kinetics. Based on the validated model and using time scale analysis with characteristic times, the optimal process and operating conditions for the developed microbioreactor system were determined. By reducing the channel thickness to 200 um and increasing the gel thickness to 900 microns, we were able to increase the complete conversion of the substrate with a residence time of less than 40 minutes. microreactor mathematical model immobilization whole-cell biocatalysis continuous biotransformation time-scale analysis mikroreaktor matematični model imobilizacija biokataliza s celimi celicami kontinuirna biotransformacija time-scale analiza true false false Angleški jezik Slovenski jezik Neznano 2023-10-20 16:46:22 2023-10-20 16:46:28 2025-04-16 12:57:26 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 biomicroreactor_excel_file.xlsx biomicroreactor_excel_file.xlsx biomicroreactor_excel_file 1 C93BA9CBDC040A9A8F128750EDDBA3DD cad7dd9048c2ec1cf92c4bc0fee95ea0a9ca1ce34331ff4834a7bc76dc2b167f bec3e938-6f58-11ee-b452-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176883 diffussion_hplc_report.pdf diffussion_hplc_report.pdf diffussion_hplc_report 1 910067F5F640C9E6DFA7693B6F2A1529 233041b9543e89ea57e94a80440f6426f0162753fe5efbfb23a497b035c36146 c7fe56ca-6f58-11ee-b452-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176884 effective-diffusivity_+_liquid-diffusivity_may-2020.xlsx effective-diffusivity_+_liquid-diffusivity_may-2020.xlsx effective-diffusivity_+_liquid-diffusivity_may-2020 1 D736460DE62E67F66796ED8BA22F62FB d2e02c8706fa11846b70df2c207d85347e3532cdce307ad4b32c55fdf8879da4 d04ec42a-6f58-11ee-b452-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176885 kinetics_2020.xlsx kinetics_2020.xlsx kinetics_2020 1 B62CFCC00256DAD0A34943F2ECBBCA9D c7510e9d9ad7553b1e72719402ebe2f3ce93891c40207eeea5cabf195d00ca90 d8d34e8e-6f58-11ee-b452-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176886 kinetics_hplc_report.pdf kinetics_hplc_report.pdf kinetics_hplc_report 1 AE8F9B382957F4A15967A77229DE100B 11f75a1cb96706702c6bce6c07580d533c2938f4dc8e2bb00e01efccc772aeb8 e4ae71a3-6f58-11ee-b452-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176887 Fakulteta za kemijo in kemijsko tehnologijo Meritve in testi 0 0 0