20.500.12556/RUL-151771 Tandem mass spectrometry de novo sequencing of the skin defense peptides of the Central Slovenian agile frog Rana dalmatina Peptides released on frogs’ skin in a stress situation represent their only weapon against micro-organisms and predators. Every species and even population of frog possesses its own peptidome being appropriate for their habitat. Skin peptides are considered potential pharmaceuticals, while the whole peptidome may be treated as a taxonomic characteristic of each particular population. Continuing the studies on frog peptides, here we report the peptidome composition of the Central Slovenian agile frog Rana dalmatina population. The detection and top-down de novo sequencing of the corresponding peptides was conducted exclusively by tandem mass spectrometry without using any chemical derivatization procedures. Collision-induced dissociation (CID), higher energy collision-induced dissociation (HCD), electron transfer dissociation (ETD) and combined MS3 method EThcD with stepwise increase of HCD energy were used for that purpose. MS/MS revealed the whole sequence of the detected peptides including differentiation between isomeric Leu/Ile, and the sequence portion hidden in the disulfide cycle. The array of the discovered peptide families (brevinins 1 and 2, melittin-related peptides (MRPs), temporins and bradykinin-related peptides (BRPs)) is quite similar to that of R. temporaria. Since the genome of this frog remains unknown, the obtained results were compared with the recently published transcriptome of R. dalmatina. agile brown frog membrane-active peptides tandem mass spectrometry top-down de novo sequencing bradykinin-related peptides BRP EThcD okretna rjava žaba membransko aktivni peptidi tandemska masna spektrometrija de novo sekvenciranje od zgoraj navzdol bradikininu sorodni peptidi BRP EThcD true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-19 13:17:49 2023-10-19 13:17:50 2023-12-05 14:01:41 0000-00-00 00:00:00 2023 0 0 14 str. iss. 20, art. 7118 Vol. 28 Oct. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2023-10-16 543.51 1420-3049 10.3390/molecules28207118 168907267 Molecules-R.Dalmatina-2023.pdf Molecules-R.Dalmatina-2023.pdf 1 EE87CD2DF081BEAF5EEFA2A1716E796E 4ea6ed71da25c3a1e1831a0184349d0d1d1047860e83213fc7b96bec42e57e8a 9f777bd9-6e71-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176829 https://www.mdpi.com/1420-3049/28/20/7118 1 24691880-6e71-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176828 Zdravstvena fakulteta 0 0 0