20.500.12556/RUL-151767 Simultaneous analysis of ascorbic and dehydroascorbic acid in fruit juice using HILIC chromatography coupled with mass spectrometry A HILIC-MS/MS method was developed for the simultaneous analysis of ascorbic acid (AA) and dehydroascorbic acid (DHA). Ammonium acetate buffer and acetonitrile were used as mobile phases under gradient elution mode at a flow rate of 0.3 mL/min. A SCIEX Triple QUAD 5500+ system was operated under MRM mode to acquire data with 175 → 115 and 173→113 as the quantifier ions for AA and DHA, respectively. Samples were prepared with a simple dilute and shoot method. A calibration and accuracy study was performed as part of method validation in fruit juices. The accuracy profiles were estimated from the measurement uncertainty determined from the validation data according to the ISO/DTS 21748. The recoveries of the analytes were between 92 – 130 %. The LOQs achieved in the current study for AA and DHA were 4.5 μg/L and 18 μg/L, respectively. These LOQs were substantially lower than what has been previously reported in the literature. The study concludes with a straightforward analytical method for the determination of total vitamin C content that does not involve timing consuming steps i.e., reduction or derivatization reaction. The method can be implemented in quality control labs for routine analysis of food samples. HPLC–MS/MS quality control multifruit juice fruit juices ascorbic acid dehydroascorbic acid accuracy profile validation sadni sok vitamini askorbinska kislina dehidroaskorbinska kislina kemična sestava HPLC-MS/MS kakovost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-19 10:36:54 2023-10-19 10:36:57 2023-12-22 12:49:59 0000-00-00 00:00:00 2023 0 0 7 str. art. 105714 Vol. 124 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543 0889-1575 10.1016/j.jfca.2023.105714 169061379 1-s2.0-S0889157523005884-main.pdf 1-s2.0-S0889157523005884-main.pdf 1 79595268A63FE8885CA0CBCAA809BBC5 0cd50b11a1553a3d6a0e7587f01980c9613cf6d41d8ff35ed52d98abcfdd413f cbd03dbd-6e5a-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176823 https://www.sciencedirect.com/science/article/pii/S0889157523005884 1 aa668c32-6e5a-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176822 Biotehniška fakulteta 0 0 0