20.500.12556/RUL-151766 Development and validation of HPLC-MS/MS method for simultaneous analysis of B vitamins present naturally or after fortification in fruit juices In this study, a multivitamin analysis method with a run time of 20 min was developed for nine major B vitamins in fruit juices using HPLC coupled with a triple quadrupole mass spectrometer. A reverse phase column with ammonium formate buffers in methanol and water as mobile phases under gradient elution mode were used to accomplish chromatographic separation. A simple dilute and shoot method was used for sample preparation. A calibration and accuracy study for method validation was conducted. The accuracy profiles were created using measurement uncertainty estimated from the validation data according to the ISO/DTS 21748. The proportional bias due to the matrix effect were ranging between 80% and 115% in the matrices investigated in this study. The measurement uncertainty and the corresponding coefficient of variation were within the acceptable limits defined in the literature and international regulatory standards i.e. EU regulation. The limits of quantitation were low enough to prove fitness for purpose for all vitamins except cyanocobalamin. The study concludes with a method with the potential to be used in quality control laboratories for high throughput routine vitamin analysis. multivitamin analysis method fruit juices accuracy profile HPLC-MS/MS quality control sadni sok vitamini kemična sestava HPLC-MS/MS kakovost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-19 10:22:55 2023-10-19 10:22:59 2024-01-03 12:56:44 0000-00-00 00:00:00 2023 0 0 9 str. art. 115103 Vol. 184 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543 0023-6438 10.1016/j.lwt.2023.115103 169054979 1-s2.0-S0023643823006825-main.pdf 1-s2.0-S0023643823006825-main.pdf 1 66D67C4A9EDCC223806E4D1BD9D90E55 6f06eb059265e2a3dbb4a6c2309d6f28369c9df4217884d3a50aa1e2a01493f7 df7ab590-6e58-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176821 https://www.sciencedirect.com/science/article/pii/S0023643823006825 1 b706ac5b-6e58-11ee-94ee-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176820 Biotehniška fakulteta 0 0 0