20.500.12556/RUL-151615 Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia The potential risk to human and animal health provides a rationale for research on methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from 283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered, most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically related isolates were identified and epidemiological data indicated transmission through, e.g., farm visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestock-associated lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes. Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices and the continuous monitoring of MRS/MRM in dairy farms. Staphylococcus aureus milk virulence factors whole genome sequencing true false true Angleški jezik Ni določen Članek v reviji 2023-10-12 13:28:11 2023-10-12 13:28:17 2023-10-13 03:41:36 0000-00-00 00:00:00 2023 0 0 19 str. issue 10, [art. no.] 1529 Vol. 10 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616:577 2079-6382 10.3390/antibiotics12101529 167972099 Genomic-Papic.pdf Genomic-Papic.pdf 1 9425312D5BD71D85E7BA1DA93AA18767 012acdb2eca03da67f5615e56872cf53fa1de40ecf498a1fdb58ff6581c81c01 da048c46-68f4-11ee-a464-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176626 https://www.mdpi.com/2079-6382/12/10/1529 1 7111eeae-68f2-11ee-a464-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176625 Veterinarska fakulteta 0 0 0