20.500.12556/RUL-151593 Immobilization of His$_6$-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes research data underlying the article Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$. nanofiber membrane microreactor biocatalysis amine transaminase enzyme immobilization membrana iz nanovlaken mikroreaktor biokataliza amin transaminaze imobilizacija encimov true false false Angleški jezik Slovenski jezik Neznano 2023-10-11 13:48:13 2023-10-11 13:48:15 2025-04-16 12:56:50 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 Data.zip Data.zip Data 1 5FA4BA21C0D1C164C62CEEAD2085AA65 6c21868125f566806d5f8c551e9643b969531a5be4ea7084703add939f1783ed 4f9902a4-6835-11ee-9b9b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176597 Direct_immobilization.xlsx Direct_immobilization.xlsx Direct immobilization 1 069EF0FA6CF278E72D4C64F76898E535 f64d2a255a2c18e3c0c103f5795a14db51f32c6719d579f1d7700d251c3b4c5c 6873a39f-6835-11ee-9b9b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176598 Nanofiber_screening.xlsx Nanofiber_screening.xlsx Nanofiber screening 1 C1A899FF5ECF864C19C7E86896079F51 c2ae11298f792efb6f273e4db56f11ddf07c984e02a9d2ba550109a027bc8deb 76c0597d-6835-11ee-9b9b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176599 Reactor_optimization.xlsx Reactor_optimization.xlsx Reactor optimization 1 889CB4CCB8A3DCA4873B80191317A8B5 f184cd128f5478e62e9ba3d08dc72482979889ccaddc8f058050ec2b04ef29b6 8c27e50a-6835-11ee-9b9b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176600 Stability_and_temperature.xlsx Stability_and_temperature.xlsx Stability and temperature 1 6FD88EA899DF58D91545F4C128A3B114 4c9ce612436e079c372258b074e8fa6ecb1d8c48483eef5c6f243b515f8c15e6 97af1a89-6835-11ee-9b9b-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176601 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0