20.500.12556/RUL-151580 Red palm weevil olfactory proteins annotated from the rostrum provide insights into the essential role in chemosensation and chemoreception Red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), is rapidly infesting palm trees (Arecaceae) in several countries, threatening coconut, date, and oil cultivations. The male-produced aggregation pheromone in palm weevils has been reported to be secreted through the mouth to the rostrum, a snout-like projection key for pheromone emission and dispersion. The olfactory mechanisms that underlie peripheral odorant detection in RPW have been addressed at the antennal level. However, the rostrum remains unexplored. Through RNA-seq, 27 odorant-binding proteins (OBPs), 6 chemosensory proteins (CSPs), 4 sensory neuron membrane proteins (SNMPs), 21 gustatory receptors (GRs), 25 odorant receptors (ORs) (including one odorant receptor coreceptor, Orco) and 10 ionotropic receptors (IRs), were identified. We reported 27 novel rostrum-specific olfactory proteins (4 IRs, 11 GRs, 2 CSPs, 3 OBPs, and 7 ORs) in R. ferrugineus (Rfer). The OBPs (RferSOBPs) [Rfer with “S” indicating “snout” (rostrum)] were the most abundant transcripts compared with the rest of the olfactory proteins. We identified several rostrum OBPs, which predominately emerged through gene duplication, and were found expressed in both rostrum and antennae. Noticeably, we found R. ferrugineus pheromone-binding protein (RferOBP1768) paralog in the rostrum (RferOBP14) and mapped it in the same scaffold at a different position on the RPW genome as a recent duplicate. We found that an OR (RferSOR1) was the most abundant for both field-collected and lab-reared RPWs, in the rostrum and antennae. Likewise, up-regulated olfactory-related proteins were established in field conditions compared with those from laboratory-reared. We found a rostrum-specific, highly expressing RferSIR1 in IR93a-clade related to hygrosensation. The role of these olfactory proteins as targets for identifying more specific and powerful semiochemicals is discussed in the context of pest management. Curculionidae palm weevil snout transcriptome aggregation pheromone olfaction chemosensory proteins palmov vrtač Rhynchophorus ferrugineus škodljivci rastlin transkriptom kemosenzorični proteini true false true Angleški jezik Slovenski jezik Članek v reviji 2023-10-10 11:32:57 2023-10-10 11:33:02 2023-10-11 03:33:51 0000-00-00 00:00:00 2023 0 0 18 str. art. 1159142 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 575.111 2296-701X 167755779 fevo-11-1159142.pdf fevo-11-1159142.pdf 1 BD489E0A0E45022F15D8B2AF059975AD 192a262056c3a6b30ef0a7dca0b010ce93e80b6637776d8942a1fb29c1264fa1 35cc4e0c-6750-11ee-9430-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176575 https://doi.org/10.3389/fevo.2023.1159142 1 045f18df-6750-11ee-9430-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176574 Biotehniška fakulteta 0 0 0