20.500.12556/RUL-151325 First molecular detection of Neospora caninum in feces of grey wolf (Canis lupus) and golden jackal (Canis aureus) populations in Slovenia Neospora caninum is an obligate intracellular parasite that causes reproductive disorders and major economic losses in cattle, and induces neuromuscular disorders in canids. Exogenous infections are becoming increasingly important due to disease outbreaks. The sylvatic life cycle of N. caninum interferes with the domestic dog-ruminant life cycle, but understanding of it is scarce. The population of wild canids may play an important role in parasite dispersion. Feces from 42 grey wolves (Canis lupus) and 39 golden jackals (Canis aureus) were analyzed for the N. caninum Nc5 gene using a novel real-time PCR (qPCR) with a detection limit of 5 targets/µL in clinical samples. Three wolves (3/42; 7.1%) and one golden jackal (1/39; 2.6%) tested positive, which is the first detection of N. caninum in the population of grey wolves in Slovenia and the first detection of N. caninum DNA in the feces of a golden jackal. In addition to the grey wolf, we propose the golden jackal as a potential definitive host with hypothetical epidemiological importance for the sylvatic-domestic life cycle of N. caninum, due to its proximity to human habitats and its rapid expansion throughout Europe. Neospora wolves jackals real-time polymerase chain reaction Neospora caninum definitive hosts grey wolf Canis lupus golden jackal Canis aureus real-time PCR qPCR digital PCR dPCR true false true Angleški jezik Ni določen Članek v reviji 2023-10-04 13:48:28 2023-10-04 13:48:31 2024-12-11 03:56:07 0000-00-00 00:00:00 2023 0 0 9 str. iss. 19, art. 3089 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:578:616 2076-2615 10.3390/ani13193089 166992131 animals-13-03089-v2.pdf animals-13-03089-v2.pdf 1 6133594A5BF28280042558D1D09B0CFC ac514002e93397f5d79027e335aac0008af4c223fac3fbd3b4dbfc800bd0e222 dcf0b43e-b6d1-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=196818 https://www.mdpi.com/2076-2615/13/19/3089 1 f1d85633-62ab-11ee-99ae-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=176274 Veterinarska fakulteta 0 0 0