20.500.12556/RUL-150971 Kožne spremembe človeške ribice Skin changes in Proteus anguinus Človeška ribica (Proteus anguinus) je ranljiva vrsta in endemit Dinarskega krasa. Poleg nedostopnosti njenega podzemnega okolja ter nezadostnega poznavanja njene razširenosti in biologije, njeno varovanje močno otežuje pomanjkljivo poznavanje njenih bolezenskih stanj. V sklopu magistrskega dela smo na histološkem nivoju proučevali kožne neoplazije, patologijo kože ob glivnih okužbah in razpadajoča tkiva najdenega trupla človeške ribice. Pri treh osebkih z neoplazijami smo prepoznali epidermalne papilome, pri enem smo spremembe opisali kot neoplazijo fibroblastov in melanoforov dermalnega izvora, pri zadnjem pa neoplazije nismo uspeli opredeliti. Pri osebkih s prepoznanimi glivnimi okužbami smo pri enem osebku prisotnost glivnih hif potrdili histološko, pri drugem z vrstično elektronsko mikroskopijo, pri tretjem pa prisotnosti hif nismo uspeli vizualizirati. Izven območja glivne razrasti nismo opazili večjih histoloških sprememb kože. Na primeru dekompozicije trupla smo opisali nivo ohranjenosti mehkih tkiv, ki so kazala na začasno zaustavljeno dekompozicijo zaradi izsuševanja. Naloga zapolnjuje vrzeli v prepoznavanju histopatoloških sprememb kože človeške ribice in predstavlja osnovo za nadaljnje natančnejše analize in razumevanje bolezenskih stanj za vzpostavitev učinkovitejšega zdravstvenega varstva te ogrožene jamske dvoživke. The European blind cave salamander, or olm (Proteus anguinus) is a vulnerable species endemic to the Dinaric Karst. In addition to the inaccessibility of its underground habitat and insufficient knowledge about its biology and distribution, the olm’s conservation efforts are facing difficulties due to a lack of understanding of its pathology. In this master’s thesis, we studied pathological changes in the skin of the olm at the histological level caused by skin neoplasia, fungal infection, and tissue decomposition. Among the individuals with neoplasia, we identified skin changes as epidermal papilloma in three individuals, dermal fibroblast and dermal cysts in one, and could not classify the neoplasm in the fifth case. In the individuals with identified fungal infections, we were able to confirm the presence of fungal hyphae histologically in one individual, by scanning electron microscopy in the second individual, and we were unable to visualize the presence of hyphae in the third individual. We did not observe any significant histological changes in the skin outside the area of fungal proliferation. In the partially decomposed specimen, we described the state of preservation of the soft tissues, suggesting that decomposition might have been temporarily suspended due to drying. The information obtained in this study hopes to fill the gaps in our understanding of pathological skin condition in the olm and provide a basis for establishing more effective health preservation measures for this endangered cave amphibian. Proteus anguinus anguinus histopatologija neoplazije glivne bolezni dekompozicija tkiv Proteus anguinus anguinus histopathology neoplasia fungal disease tissue decomposition true false false [L. Vodnik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-09-26 09:12:36 2023-09-26 09:12:37 2023-10-20 08:24:20 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 597.92(043.2) 231634 169195779 35331.pdf 35331.pdf 1 DEDC9CE2C8370B05C176E8F260E50969 cc4fb7aae2aabf3d62078b6a5845592e639579ead625875e7fefbbd0679855e3 12eae2a7-5c3c-11ee-9c9a-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=175846 Biotehniška fakulteta 0 0 0