20.500.12556/RUL-150024 Dinamika strukture in delovanja telomer v nesmrtni človeški celični liniji Dynamics of telomere structure and function in an immortalized human cell line Telomere so končna zaporedja v kromosomski DNA, ki jo ščitijo pred poškodbami in mutacijami. Poleg tega jo ščitijo pred krajšanjem med procesom duplikacije. Zaradi te naloge je njihovo delovanje povezano s staranjem ali senescenco. Dodajanje telomer uravnava encim telomeraza, ki pa preneha delovati v večini odraslih celic zaradi evolucijskega mehanizma, ki ščiti pred rakom. Nesmrtne celične linije izkoriščajo telomerazo za njihovo neprenehno proliferacijo, zato je proučevanje telomer zelo pomembno za razumevanje nesmrtnih celičnih linij. V okviru svojega diplomskega dela bom opisal strukturo in funkcijo telomer. S pregledom literature, ki temelji na primerih nesmrtnih celičnih linij, bom raziskal dinamiko telomer. Telomeres are end sequences in chromosomal DNA that protect it from damage and mutations. In addition, they protect DNA from shortening during the replication process. Due to this task, their activity is related to aging or senescence. The addition of a telomere is performed by the enzyme telomerase, but it ceases to function in most adult cells due to an evolutionary mechanism that protects against cancer. Immortal cell lines take advantage of telomerase for their continuous proliferation. Therefore, the study of the telomere is very important for understanding immortal cell lines. As part of my thesis, I will describe the structure and function of the telomere. I will explore telomere dynamics through a literature review using several examples of immortalized cell lines. telomere telomeraza nesmrtne celične linije preslikava po Southern-u kromosomska fuzija polimorfizem telomeres telomerase immortal cell lines Southern blot chromosomal fusion polymorphism true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-13 08:15:57 2023-09-13 08:16:01 2023-09-15 11:37:07 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 229178 164590083 35248.pdf 35248.pdf 1 D492DA9ECE9F721FC44AEB300B5CC02E 2ca7319436f2a1fbe7860ed97f5e3e3a7aa9434cd994ad8ee95d87d910c6d55b 02d75438-51fd-11ee-916e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=174556 Biotehniška fakulteta 0 0 0