20.500.12556/RUL-149780 Cirkadiani ritem lokomotorne aktivnosti vodnega oslička (Asellus aquaticus) Circadian rhythm of locomotor activity in the freshwater isopod Asellus aquaticus Cirkadiani ritem je biološki ritem endogenega izvora, ki se ponavlja s periodo približno 24 ur. Cirkadiani ritem se pri rakih pogosto odraža z lokomotorno aktivnostjo. V magistrski nalogi smo želeli analizirati cirkadiano lokomotorno aktivnost površinskih vodnih osličkov (Asellus aquaticus) in vzpostaviti eksperimentalni sistem in protokol za analize evolucije cirkadianih ritmov te vrste. Živali smo nabrali na Planinskem polju in jih aklimirali v laboratoriju. Nato smo vodne osličke posamič prestavili v petrijevke in jih sedem dni snemali v svetlobnemu režimu 12h:12h LD, nato pa še sedem dni v stalni temi, 24h DD. Video posnetke smo analizirali s tehniko video sledenja. Lokomotorno aktivnost smo prikazali s standardnim aktogramom in drugimi grafi. Na podlagi rezultatov domnevamo, da vodni oslički nimajo izražene cirkadiane ritmike lokomotorne aktivnosti. Domnevna aritmičnost površinskih vodnih osličkov je morda eden izmed razlogov za njihovo večkratno uspešno kolonizacijo podzemlja. Osebki okuženi s parazitom ježerilcem Acanthocephalus anguillae so bili lokomotorno aktivnejši kot neokuženi osebki. Domnevamo, da je zato prenos parazita iz vmesnega (oslički) v končnega gostitelja (ribe) verjetnejši. Izvedeni poskus ima nekaj metodoloških pomanjkljivosti (npr. stradanje živali, mehanske motnje), zato so za potrditev zgornjih domnev potrebna dodatna testiranja. The circadian rhythm is a biological rhythm of endogenous origin that repeats with a period of approximately 24 hours. Circadian rhythm in crustaceans is often expressed in locomotor activity. The goal of this study was to analyze the circadian locomotor activity of surface-dwelling water louse (Asellus aquaticus) and establish an experimental system and protocol for the analysis of the evolution of circadian rhythms of this species. We collected animals at Planina Polje and acclimatized them in the laboratory. Then we transferred them individually into Petri dishes and recorded their behavior in 12h:12h LD light regime for seven days, and then in constant darkness, 24h DD, for another seven days. Videos were analyzed using video-tracking techniques. Locomotor activity was shown with a double-plotted actogram and other graphs. Based on the results, we assume that water louse does not have a circadian rhythm of locomotor activity. Their alleged arrhythmicity may be one of the reasons for their multiple successful colonizations of subterranean habitats. Additionally, individuals infected with the parasite Acanthocephalus anguillae had greater locomotor activity than uninfected individuals. We assume this increases the probability of parasite’s transfer from the intermediate (water louse) to the final (fish) host. The conducted study includes potential methodological flaws (e.g., animal starvation, mechanical disturbances), so further research is needed to confirm the above assumptions. lokomotorna aktivnost cirkadiani ritem aktogram vodni osliček Asellus aquaticus locomotor activity circadian rhythm actogram water louse Asellus aquaticus true false false [Š. Škof] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-09-10 07:16:29 2023-09-10 07:16:33 2023-09-12 03:21:14 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 57:595.3(043.2) 228935 163965187 34401.pdf 34401.pdf 1 42D11DF029E25331FD8EE795692565ED 4a67fe9e0fc0f401d771ca12ec439f7e3ac7da84c2d7f9807832e440df7bd139 34f65f8e-4f99-11ee-a2ce-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=174281 Biotehniška fakulteta 0 0 0