20.500.12556/RUL-149555 Spektroskopske raziskave vezanja ligandov na DNK Spectroscopic studies of ligand binding to DNA Spektroskopske raziskave vezanja ligandov na DNK temeljijo na merjenju spremembe spektroskopskih lastnosti raztopine vzorca, ki je posledica same vezave. Tako lahko s pomočjo UV-Vis spektroskopije izmerimo absorpcijske UV-Vis spektre pri vezavi ter s pomočjo Scatchardovega diagrama določimo jakost in stehiometrijo vezave liganda na DNK. V diplomski nalogi sem proučila vezavo ligandov na molekulo naravne dvoverižne DNK iz govejega priželjca. Izbrani ligandi so bili DAPI, akridin oranžno in metilen modro. Analiza potrjuje, da se ligandi vežejo na DNK na podoben način, in sicer v ožji kanal DNK, pri čemer pomembno vlogo igrajo elektrostatske interakcije med molekulo liganda in DNK. Vsem ligandom je skupno tudi to, da se interkalirajo med bazne pare v DNK, kar pa smo uspeli pokazati samo za ligand metilen modro. Spectroscopic studies of ligand binding to DNA are based on measuring the change in the spectroscopic properties of the sample solution resulting from the binding itself. Thus, with the help of UV-Vis spectroscopy, we can measure the absorption UV-Vis spectra during binding, and with the help of the Scatchard diagram, we can determine the strength and stoichiometry of the binding of the ligand to DNA. In my diploma thesis, I studied the binding of ligands to a molecule of natural double-stranded DNA from bovine sputum. The selected ligands were DAPI, acridine orange and methylene blue. The analysis confirms that ligands bind to DNA in a similar way, namely in a narrower DNA channel, with electrostatic interactions between the ligand molecule and DNA playing an important role. All ligands also have in common that they intercalate between base pairs in DNA, which we were able to demonstrate only for the ligand methylene blue. deoksiribonukleinska kislina Scatchardova analiza spektroskopija vezava liganda deoxyribonucleic acid Scatchard analysis spectroscopy ligand binding true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-07 11:27:10 2023-09-07 11:27:10 2023-09-15 10:15:30 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 22730 164553475 Crnkic_Ajla_-_Spektroskopske_raziskave_vezanja_ligandov_na_DNK.pdf Crnkic_Ajla_-_Spektroskopske_raziskave_vezanja_ligandov_na_DNK.pdf 1 FBA8B1176CBADA6D29C2B9872AA13431 51f39dd06049901080c2b818622a2363d70a431aa25e05ebbecf65cd762a3753 b74fe80d-4d60-11ee-9934-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=174039 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0