20.500.12556/RUL-149250 Tvorba citokinov po spodbujanju makrofagov z različnimi sevi streptokokov skupine B Production of cytokines in response to macrophage stimulation with different strains of Group B Streptococcus Streptokoki skupine B (GBS) so vodilni vzrok za bolezni in smrt novorojenčkov po vsem svetu. Prav tako imajo pomemben vpliv na nosečnice, starejše in imunsko oslabljene osebe. Prehod iz asimptomatske kolonizacije v invazivno okužbo še ni povsem razumljen. V naši raziskavi smo s pomočjo pretočnega citometra analizirali tvorbo citokinov po stimulaciji makrofagov, da bi raziskali vpliv različnih sevov GBS na potek in resnost bolezni. Ugotovili smo razlike v tvorbi vnetnih in regulatornih citokinov ter kemokinov 3 in 24 ur po stimulaciji makrofagov ter razlike v tvorbi citokinov med posameznimi serotipi GBS. Opazili smo, da na profil citokinov vpliva različna citotoksičnost posameznih sevov. Na podlagi meritev IL-1ß in IL-18 predvidevamo, da določeni sevi GBS povzročajo piroptozo v stimuliranih makrofagih. Z analizo tvorbe IL-12 smo ugotovili tudi, da prihaja do razlik v aktivaciji sekundarnega imunskega odziva med sevi GBS. Pri hipervirulentnem sekvenčnem tipu ST-17 pa smo ugotovili nižjo produkcijo vnetnih citokinov na začetku okužbe ter povišano tvorbo regulatornega citokina IL-10. To povezujemo z njegovo sposobnostjo izmikanja imunskemu sistemu in povzročanjem invazivnih okužb. Group B Streptococcus (GBS) is one of the most common causes of neonatal illness and death worldwide. Pregnant women, the elderly, and immunocompromised individuals are also severely affected by GBS. The transition from asymptomatic colonization to invasive disease is not yet fully understood. In our study, we used flow cytometry to analyze the production of cytokines after stimulation of macrophages to investigate the effects of different GBS strains on the severity of disease progression. We found differences in the production of inflammatory cytokines, regulatory cytokines and chemokines 3 and 24 hours after macrophage stimulation, as well as differences in cytokine production between different GBS serotypes. We observed the influence of different cytotoxic GBS strains on the profile of cytokine production. Based on the measurements of IL-1ß and IL-18, we hypothesize that certain GBS strains induce pyroptosis in stimulated macrophages. By analyzing IL-12 production, we found differences in activation of the secondary immune response between GBS strains. In the case of the hypervirulent sequence type ST-17, we observed lower production of inflammatory cytokines and increased production of the regulatory cytokine IL-10 at the onset of infection, which we related to its ability to evade the immune system and cause invasive infections. streptokoki skupine B makrofagi citokini pretočna citometrija group B Streptococcus macrophages cytokines flow cytometry true false false [S. Petrin] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2023-09-06 07:15:10 2023-09-06 07:15:18 2023-10-10 10:15:11 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.2:579.862:577.27 228352 163433731 34449.pdf 34449.pdf 1 D76A676D2AB62273D6C771C2608C0433 73beebbe34e0711fadb8ebfefc6b14898c0017b5412ec0c62c6a4a1c9e7d581d 64ca5a47-4c74-11ee-af86-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=173680 Biotehniška fakulteta 0 0 0