20.500.12556/RUL-149225 Proučevanje raznolikosti sevov vrste Hortaea werneckii različnih ploidnosti v odvisnosti od temperature in koncentracije NaCl Study of the diversity of Hortaea werneckii strains of variable ploidity depending on temperature and salinity Hortaea werneckii je melanizirana meristematska askomicetna gliva rodu Capnodiales. Poznamo jo predvsem kot etiološki agens humane okužbe tinea nigra in kot pomemben evkariontski modelni organizem halotolerance. Omenjeno črno kvasovko odlikuje zmožnost preživetja tako v celotnem razponu koncentracij NaCl (od 0–30 %) kot v prisotnosti nekaterih drugih kaotropnih soli. Njena primarna ekološka niša so solni bazeni solin ter druge slane vode in grenčice, bogate z Mg solmi. V sklopu diplomskega dela smo analizirali rast 16 sevov H. werneckii različnih ploidnosti (n in 2n) v gojišču brez NaCl ter z dodatkom 17 % NaCl . Celice vzgojenih kultur smo morfološko opisali in njihovo velikost izmerili pod svetlobnim mikroskopom. Pridobljene rezultate smo obdelali z različnimi statističnimi metodami – opisno statistiko, PCA, t testom in Wilcoxovim testom. Ob primerjavi rasti različnih sevov H. werneckii glede na slanost gojišča smo določili, da je bilo v gojišču brez dodane soli več celic prisotnih v obliki kvasovk, medtem ko je bilo v gojišču s 17 % NaCl več hif in psevdohif. Prav tako smo v izredno slanem gojišču opazili več širših konidijev kot v gojišču brez soli. Haploidni sevi so pogosteje oblikovali enocelične konidije kot diploidni sevi. Slednji so tvorili krajše in ožje eno- ter dvocelične konidije v primerjavi s haploidnimi sevi. Pogosteje so rasli v obliki hif in psevdohif, kar kaže na večjo prilagojenost diploidnih sevov na izredno slano okolje. Hortaea werneckii is a melanized meristematic ascomycetous fungus of the genus Capnodiales. It is known primarily as the etiological agent of the human mycotic infection tinea nigra and as an important eukaryotic model organisms for the study of halotolerance. This black yeast is characterized by its ability to survive in the entire range of NaCl concentrations, from 0 to 30 % NaCl, as well as in the presence of some other chaotropic salts. It’s primary ecological niche are salt pans, other saline waters and bitterns, waters rich with Mg salts. In this diploma thesis we analysed the growth of several strains of H. werneckii of differentiated ploidies (n and 2n) grown in media without NaCl and with 17 % NaCl. We observed the morphology and measured the size of cultured cells under a light microscope. The obtained results were analysed with various statistical methods, including descriptive statistics, PCA, t test and Wilcox test. By comparing the growth of different strains of H. werneckii in media of different salinity, we determined that in the medium without added salt, there were more yeast cells present, whereas in the medium with 17%NaCl we noticed more hyphae and pseudohyphae. Additionally, we observed wider conidia in the hypersaline medium compared to the medium without salt. Haploid strains more frequently formed unicellular conidia than diploid strains. The latter grew shorter and narrower one- and two-celled conidia compared to the haploid strains. They also exhibited a greater tendency to grow in the form of hyphae and pseudohyphae, indicating a higher adaptation of diploid strains to the extremely salty environment. Hortaea werneckii črna kvasovka ekstremofili halofili ploidnost Hortaea werneckii black yeast extremophiles ploidy halophile true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-05 12:05:00 2023-09-05 12:05:04 2023-10-09 12:57:32 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 21881 167334659 Vene_Eva_-_Proucevanje_raznolikosti_sevov_vrste_Hortaea_werneckii_razlicnih_ploidnosti_v_odvisno.pdf Vene_Eva_-_Proucevanje_raznolikosti_sevov_vrste_Hortaea_werneckii_razlicnih_ploidnosti_v_odvisno.pdf 1 3EB39233F495B493D5D85E2FF2E93780 82523611f471378f99d581817881038db144615745444115d05b2e126ca55eb4 af381ad5-4bd3-11ee-af86-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=173651 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0