20.500.12556/RUL-149087 Virusne majhne molekule RNA kot modulatorji izražanja genov gostiteljske rastline Virus derived small RNAs as modulators of plant host gene expression Kompleksne interakcije med virusi in gostiteljskimi rastlinami, zlasti v kontekstu vsiRNA, predstavljajo večplastno področje preučevanja. V tem diplomskem delu je raziskana zapletena dinamika vsiRNA, majhnih virusnih molekul RNA, ki jih rastline prvotno proizvajajo za obrambo pred virusno RNA ali DNK, vendar se lahko paradoksalno obrnejo proti gostitelju z vezavo na njegove transkripte. Ta diplomska naloga razkriva, da so številni ciljni gostiteljski transkripti sestavni del bistvenih rastlinskih funkcij, kot so biosinteza klorofila, sinteza transkripcijskih faktorjev, imunski sistem in različni presnovni procesi. Opisani primeri prikazujejo, kako prihaja do interakcij med tarčnimi transkripti v tobaku in pšenici, kar povzroči simptome, ki spominjajo na virusno okužbo, ter zapletene interakcije v bombažu, kjer so tarče potencialni transkripti MYB, in podobno delovanje v rižu, kjer so tarče vsiRNA beljakovine, kot sta DCL in AGO. Proučene so bile tudi interakcije med vsiRNA in dolgimi nekodirajočimi RNA, kjer vsiRNA utišajo izražanje lncRNA in s tem okrepijo simptome virusne okužbe. Raziskava pušča odprto vprašanje, kakšen je mehanizem, ki se skriva za vdorom virusov v sistem RNAi in zapletenim vključevanjem pomembnih delov rastlinskega genoma v genom virusa, kar olajša nadaljnje širjenje virusa. The complex interactions between viruses and host plants, particularly in the context of vsiRNA, present a multifaceted area of study. This research explores the intricate dynamics of vsiRNA, small viral RNA molecules that are originally produced by plants for defense against viral RNA or DNA but may paradoxically turn against the host by binding to its transcripts. This investigation reveals that many target host transcripts are integral parts of essential plant functions, such as chlorophyll biosynthesis, transcription factors synthesis, immune system and various metabolic processes. Specific cases illustrate how transcripts in tobacco and wheat are targeted, resulting in symptoms reminiscent of a viral infection, along with intricate interactions in cotton that target potential MYB transcripts, and similar actions in rice that target proteins such as DCL and AGO. The research also delves into the interactions between vsiRNAs and long non-coding RNAs, where the vsiRNAs silence the expression of lncRNAs, thereby amplifying the symptoms of the viral infection, adding another layer to the intricate mechanisms by which viruses exploit host systems. The thesis leaves open the question of the underlying mechanism behind the hijacking of the RNAi system by viruses and the intricate incorporation of significant regions of the plant genome into the viral genome to facilitate virus spread. vsiRNA rastline rastlinski virusi RNA interferenca modulacija izražanja genov odpornost na bolezni interakcije med rastlinami in virusi post-transkripcijsko gensko utišanje vsiRNA plants plant viruses RNA interference modulation of gene expression disease resistance plant-virus interactions post-transcriptional gene silencing true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-03 07:16:24 2023-09-03 07:16:26 2023-09-05 09:08:29 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 228231 163261187 34584.pdf 34584.pdf 1 7650AB0DBFE673C0F30EED41526F45EF 498492e886930ce3c598a0fa49da88588dbcbeb4c0cb71f3023ff54ded1314ef 065f0b0f-4a19-11ee-af86-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=173474 Biotehniška fakulteta 0 0 0