20.500.12556/RUL-148341 Metabolite diversity in pulp segments, peel, leaves, and bark of a red-fleshed ‘Baya Marisa’ apple cultivar This study investigated the composition of sugars, organic acids, individual and total phenolic compounds in the pulp, peel, leaves, and bark of a red-fleshed ‘Baya Marisa’ apple cultivar. As the fruit is known for its red pulp color, the study focused on comparing the profiles of primary and selected secondary metabolites in three sections along the equatorial fruit plane. The analyses were carried out using HPLC–MS system, and compositional differences were compared among fruit segments. Inner fruit segments accumulated higher levels of sorbitol and the sum of sugars and lower levels of citric acid. However, no differences in the sum of organic acids could be determined among segments. The phenolic composition differed among pulp (hydroxycinnamic acids > dihydrochalcones ≈ anthocyanins ≈ flavanols > flavonols), peel (flavanols > anthocyanins > dihydrochalcones > flavonols > hydroxycinnamic acids), leaves (flavonols > dihydrochalcones > hydroxycinnamic acids > anthocyanins), and bark (dihydrochalcones > flavonols > flavanols > anthocyanins > hydroxycinnamic acids). The greatest phenolic diversity was detected in bark (25), followed by leaves (18), peel (17) and finally, pulp (11). Three anthocyanins (cyanidin-3-O-galactoside > cyanidin-3-O-arabinoside > peonidin-3-O-galactoside) were determined in all ‘Baya Marisa’ tissues with their content highest in the peel. The innermost sections of the fruit were characterized by higher levels of dihydrochalcones and lower levels of most flavanols, flavonols and anthocyanins. These were predominant in the apple pulp nearest to the peel, with cyanidin-3-O-galactoside being the prevalent representative. Accumulation of anthocyanins in pulp is a rare trait in apples, and therefore, the distinct distribution and diversity of metabolites in this cultivar highlights its potential for high-nutrient products such as juices, apple chips or purees. Malus domestica peel leaves bark pulp phenolics anthocyanins secondary metabolites jabolka pulpa olupek listi lubje sekundarni metaboliti fenolne spojine antocianini true false true Angleški jezik Slovenski jezik Članek v reviji 2023-08-17 12:45:52 2023-08-17 12:45:56 2024-12-06 12:12:38 0000-00-00 00:00:00 2023 0 0 15 str. iss. 8, art. 1564 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.1/.7 2077-0472 10.3390/agriculture13081564 161496323 agriculture-13-01564-v2.pdf agriculture-13-01564-v2.pdf 1 CEA322ABC036B9612A1470FDC754BFBD 5cfde0c5c9612638d10083470187a6b0b57f7ec9c254d0b7db64ef7bb61d2230 7b1122ff-3ceb-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172473 https://www.mdpi.com/2077-0472/13/8/1564 1 3b14e783-3ceb-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172472 Biotehniška fakulteta 0 0 0