20.500.12556/RUL-148332 Rehoming and other refinements and replacement in procedures using golden hamsters in SARS-CoV-2 vaccine research Effective vaccines are needed to fight the COVID-19 pandemic. Forty golden hamsters were inoculated with two promising vaccine candidates and eighteen animals were used in pilot trials with viral challenge. ELISA assays were performed to determine endpoint serum titres for specific antibodies and virus neutralisation tests were used to evaluate the efficacy of antibodies. All tests with serum from vaccinated hamsters were negative even after booster vaccinations and changes in vaccination protocol. We concluded that antibodies did not have sufficient neutralising properties. Refinements were observed at all steps, and the in vitro method (virus neutralisation test) presented a replacement measure and ultimately lead to a reduction in the total number of animals used in the project. The institutional animal welfare officer and institutional designated veterinarian approved the reuse or rehoming of the surplus animals. Simple socialization procedures were performed and ultimately 19 animals were rehomed, and feedback was collected. Recently, FELASA published recommendations for rehoming of animals used for scientific and educational purposes, with species-specific guidelines, including mice, rats, and rabbits. Based on our positive experience and feedback from adopters, we concluded that the rehoming of rodents, including hamsters, is not only possible, but highly recommended. vaccines mesocricetus models animals SARS-CoV-2 vaccine research golden hamster animal models biokemija covid-19 koronavirusi cepljenje virusi okužbe cepiva true false true Angleški jezik Slovenski jezik Članek v reviji 2023-08-16 14:25:15 2023-08-16 14:25:20 2024-12-10 11:24:39 0000-00-00 00:00:00 2023 0 0 16 str. iss. 16, art. 2616 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:578 2076-2615 10.3390/ani13162616 161400579 Rehoming-Strbenc.pdf Rehoming-Strbenc.pdf 1 F3BCC75B5BDE711662C53F04F9171E69 27a3f60b71172813eb0f70e04ead490d22e67d92550ce42eedf706679840c5b5 2ff03b9a-3c31-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172454 https://www.mdpi.com/2076-2615/13/16/2616 1 f3ce87c3-3c2f-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172453 Veterinarska fakulteta 0 0 0