20.500.12556/RUL-148279 Proteomics of the haemolymph of the terrestrial crustacean Porcellio scaber reveals components of its innate immunity under baseline conditions The terrestrial crustacean Porcellio scaber is an established test organism in environmental research. We analysed the haemolymph proteome of P. scaber using a classical proteomic approach based on one-dimensional gel electrophoresis and tandem mass spectrometry. Using a publicly available protein database and our P. scaber transcriptome data, we have identified 76 proteins involved in cytoskeleton formation, protein degradation, vesicular transport, genetic information processing, detoxification, carbohydrate and lipid metabolism reflecting haemocyte metabolic activity, active intracellular transport, and intercellular communication. Compared with the data reported for other crustaceans, 28 of these P. scaber proteins have been linked to its immunity, among them hemocyanin, α-2-macroglobulin, phenoloxidase 3, superoxide dismutase, glutathione S-transferase, haemolymph clottable protein, and histones H4 and H2B. Our results thus provide a firm base for studying the innate immune response of P. scaber at the level of the haemolymph proteome. This knowledge is of particular importance in ecotoxicity studies with various environmental stressors where understanding physiological changes is important to reveal possible modes of action. woodlice Porcellio scaber haemolymph proteome immunity mass spectrometry true false true Angleški jezik Ni določen Članek v reviji 2023-08-09 13:00:14 2023-08-09 13:00:16 2023-08-10 03:36:57 0000-00-00 00:00:00 2023 0 0 Str. 12-21 Vol. 213 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 0300-9084 10.1016/j.biochi.2023.05.007 152020483 1-s2.0-S0300908423001207-main.pdf 1-s2.0-S0300908423001207-main.pdf 1 36CDCAF67AE922011FECA94B752231B0 430cd0b042e19e4ebe2ba19a59c808304bfff0a4c0aa77db6e2b036fa5f155fa 2bfd43bd-36a4-11ee-aeaf-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172362 https://www.sciencedirect.com/science/article/pii/S0300908423001207 0 f2cc4f61-36a3-11ee-aeaf-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172361 Biotehniška fakulteta 0 0 0