20.500.12556/RUL-147614 Vpliv ampicilina na obstojnost biofilma bakterije Escherichia coli Effect of ampicillin on formed biofilm of Escherichia coli Biofilmi so združbe bakterijskih celic, ki živijo pritrjene na podlago v vlažnih okoljih. Znotraj združbe prihaja tako do sodelovanja kot do izkoriščanja med bakterijami. Celice so med seboj povezane z zunajceličnim matriksom, ki omogoča komunikacijo, prav tako pa pripomore k zaščiti pred zunanjimi vplivi, imunskim sistemom in protimikrobnimi sredstvi. Celice imajo tudi upočasnjen metabolizem in spremenjeno izražanje genov, kar le še pripomore k težavni odstranitvi okužb z biofilmi. Ker le te predstavljajo velik problem v zdravstvu, je bistvenega pomena poznavanje odziva biofilmov na terapije z antibiotiki. V diplomskem delu smo vzpostavili bioreaktorski sistem, v katerem smo različno dolgo tvorili biofilme bakterije Escherichia coli ter jih spirali z dvema različnima koncentracijama ampicilina v SM pufru. Tekom daljšega časa spiranja se je dovzetnost bakterij za antibiotik nižala. Višja koncentracija ampicilina je imela večji vpliv tako na biofilme kot na planktonske celice. Tudi čas tvorjenja biofilma je imel vpliv na kočno koncentracijo celic, saj so bile pri daljšem nalaganju pri vseh vzorčenjih gostote celic višje. Biofilms are communities of bacterial cells that live attached to a surface in moist environments. Within a community, both cooperation and exploitation can occur. Cells are connected to each other with extracelullar matrix that enables communication and at the same time protects cells against external influences, the immune system and antimicrobial agents. Cells in biofilms have slower metabolism and altered gene expression, which contribute to the difficult removal of biofilm infections. The latter represent a major problem in healthcare, so it is essential to understand the responses of biofilms to antibiotic therapy. In our thesis, we formed Escherichia coli biofilms in coiled bioreactor and tested how different loading times as well as different concentrations of antibiotic ampicilin in SM buffer affect biofilms and planktonic cells. During the rinsing of biofilms, the susceptibility to the antibiotic decreased. We showed that a higher concentration of ampicilin has a greater effect on both biofilms and planktonic cells. Longer loading time had an impact on the final concentration of cells, as the cell densities were higher with longer loading period. biofilm E. coli odpornost na antibiotike cevni bioreaktor biofilm E. coli resistance to antibiotics coil bioreactor true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-07-10 07:16:06 2023-07-10 07:16:13 2023-07-11 03:37:46 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 218935 158346243 33119.pdf 33119.pdf 1 57D993F6504FD2F803FED4B39729303E 49f3da2d9e9ca436fa5c5d3be410825773e6affa991befe341fe08ce61c778ce 74452ad4-1ee0-11ee-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=171268 Biotehniška fakulteta 0 0 0