20.500.12556/RUL-147544 Discovery of a novel bat lyssavirus in a long-fingered bat (Myotis capaccinii) from Slovenia Lyssaviruses are the causative agents of rabies, a zoonotic, fatal disease that is thought to be ancestral to bats. In the last decade, the detection of bat associated lyssaviruses is increasing also in Europe. Within a retrospective bat associated lyssavirus surveillance study a total of 225 dead bats of 21 bat species were collected in Slovenia between 2012 and 2019 and tested by specific real-time RT-PCR method. The first lyssavirus positive sample in bats in Slovenia was detected using the real-time RT-PCR, the fluorescent antibody test, and next generation sequencing, while the rabies tissue culture inoculation test was unsuccessful due to sample degradation and storage conditions. The nearly complete genome of Divača bat lyssavirus from Slovenia consists of 11,871 nucleotides and reflects the characteristic gene organization known for lyssaviruses, encoding the five viral proteins. Phylogenetic analysis of Divača bat lyssavirus revealed that it belongs to phylogroup I lyssaviruses and is most closely related to Kotalahti bat lyssavirus (KBLV) with 87.20% nucleotide and 99.22% amino acid identity. Together with KBLV, Khujand virus, European bat lyssavirus 2, Bakeloh bat lyssavirus, and Aravan virus, Divača bat lyssavirus was detected in the genus Myotis suggesting its key role in the transmission and maintenance of certain lyssaviruses. real-time polymerase chain reaction lyssaviruses bats rabies virus phylogenetic analysis Europe zoonoses nucleotides species delimitation true false true Angleški jezik Ni določen Članek v reviji 2023-07-07 10:24:36 2023-07-07 10:24:41 2024-08-27 15:11:55 0000-00-00 00:00:00 2023 0 0 12 str. iss. 6, art. e0011420 Vol. 17 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09.578 1935-2735 10.1371/journal.pntd.0011420 157448451 Discovery-Kuhar.pdf Discovery-Kuhar.pdf 1 6DF547344A8B328BDDADAC72F45B161D bed95fcb24686f8bb9ab4a2fc6092ae35613bc429623df8b0c1ed2ad4d009b28 e68a7bf2-1c9f-11ee-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=171163 https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011420 1 4d180360-1c9f-11ee-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=171162 Veterinarska fakulteta 0 0 0