20.500.12556/RUL-147076 Salinity inhibition in thermophilic anaerobic digestion of organic waste Anaerobic digestion, despite its preferable use as a treatment for high organic matter polluted waste streams, is susceptible to inhibitors, salt included. Therefore, two different experiments were conducted to observe the responses of bacterial and archaeal communities to hypersaline environments. In the first experiment, salt was added gradually, while in the second experiment, salt was added rapidly (so-called salt shocks were performed). The results of the gradual addition of salt showed a recovery of methane production after the salt concentration decreased. The NaCl concentration of 28.2 g/L seems to be the limit between stable operation and occurrence inhibition. The specific biogas production varied between 0.490 and 0.562 m3/kgtCOD during the stepwise salt addition, depending on the salt concentration, while the maximal achieved COD removal was 79.8%. The results of the rapid salt addition showed good recovery of the bacterial community, while a reduction of salt-sensitive species was observed in the archaeal community. The trend of specific biogas production during rapid salt addition was stable with an average value of 0.590 m3/kgtCOD, and it was observed that higher concentrations of up to 39.4 g/L of NaCl were tolerated. The maximum COD removal achieved during rapid salt addition was 83.1%. In conclusion, certain bacterial and archaeal communities were well-adapted to the hypersaline environment and remained active during the anaerobic digestion of substrates with high salt concentration. biogas organic waste microbial community structure terminal restriction fragment length polymorphism salinity inhibition thermophilic anaerobic digestion mikrobiologija organski odpadki bioplin anaerobna razgradnja slanost true false true Angleški jezik Slovenski jezik Članek v reviji 2023-06-22 13:47:29 2023-06-22 13:47:30 2023-06-23 03:41:09 0000-00-00 00:00:00 2023 0 0 15 str. iss. 11, art. 6590 Vol. 13 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-3417 10.3390/app13116590 153890051 applsci-13-06590.pdf applsci-13-06590.pdf 1 D9AB8DCB0C3947444C6BF20E763CC53E a04ce756bff353e4d95124da377abd9ef316aa002441f5310a7c53a830f5a5aa 3d9869dc-10f3-11ee-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=170430 https://www.mdpi.com/2076-3417/13/11/6590 1 3129b56e-10f2-11ee-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=170428 Biotehniška fakulteta 0 0 0