20.500.12556/RUL-146423 Meeting in Liguria hybridisation between Apennine endemic Euphorbia barrelieri and western Mediterranean E. nicaeensis led to the allopolyploid origin of E. ligustica The Mediterranean Basin is renowned for its extremely rich biota and is considered as one of the 25 Global Biodiversity Hotspots, but its diversity is not homogeneously distributed. Outstanding in the number of (endemic) species are the Ligurian Alps (Italy). At the foot of the Ligurian Alps, little above the Mediterranean Sea, a disjunct occurrence of Italian endemic Euphorbia barrelieri was reported. Using an array of integrative methods ranging from cytogenetic (chromosome number and relative genome size estimation), over phylogenetic approaches (plastid, ITS and RAD sequencing) to multivariate morphometrics we disentangled the origin of these populations that were shown to be tetraploid. We performed phylogenetic analyses of the nuclear ITS and plastid regions of a broad taxonomic sampling of Euphorbia sect. Pithyusa to identify possible species involved in the origin of the tetraploid populations and then applied various analyses of RADseq data to identify the putative parental species. Our results have shown that the Ligurian populations of E. barrelieri are of allotetraploid origin that involved E. barrelieri and western Mediterranean E. nicaeensis as parental species. We thus describe a new species, E. ligustica, and hypothesise that its adaptation to similar environments in which E. barrelieri occurs, triggered development of similar morphology, whereas its genetic composition appears to be closer to that of E. nicaeensis. Our study emphasises the importance of polyploidisation for plant diversification, highlights the value of the Ligurian Alps as a hotspot of biodiversity and endemism and underlines the importance of integrative taxonomic approaches in uncovering cryptic diversity. integrative taxonomy Ligurian Alps morphometrics new species phylogenetics polyploidy RAD sequencing taksonomija Ligurske alpe morfometrija nove vrste filogenetika poliploidija sekvenciranje true false true Angleški jezik Slovenski jezik Članek v reviji 2023-05-31 14:01:44 2023-05-31 14:01:48 2023-06-15 11:19:28 0000-00-00 00:00:00 2023 0 0 15 str. Vol. 185 art. no. ǂ107805 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 58 1055-7903 10.1016/j.ympev.2023.107805 154027267 1-s2.0-S1055790323001057-main_(1).pdf 1-s2.0-S1055790323001057-main_(1).pdf 1 6E1EAB360FE36D85E5CED5D3B5CE1F59 04adb6e079ee9efae971045bc81f8e6f6923d37cb1eb295e36d679e7b52c7c18 512daefe-ffb4-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=169625 https://www.sciencedirect.com/science/article/pii/S1055790323001057 1 9dd04feb-ffaa-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=169618 Biotehniška fakulteta 0 0 0