20.500.12556/RUL-145905 ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains a review Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chemical, and physical composition of the skeletal tissue, and the quality and quantity of DNA preserved in the tissue, also considering the skeletal element, absolute age, and environmental conditions to which the remains have been exposed. Our research focuses on human bones and teeth derived from archaeological, WWII and forensic contexts. The samples are cleaned and pulverized according to established methodological procedures for DNA extraction. DNA is extracted and quantified, while the remaining powder is analysed by ATR-FTIR spectroscopy. The obtained spectra are manipulated to extract the chemometric indices, which are the base for statistical analyses, machine learning, and prediction algorithms. A compilation of results from several studies shows that ATR-FTIR spectra present a solid base for researchers to better understand the preservation state of phosphates, carbonates, and collagen in the skeletal tissues, assessment of post-mortem interval, and the preservation of the DNA. Although many questions still need to be answered, ATR-FTIR spectroscopy with further data manipulation has a high potential for research of taphonomic changes, PMI and DNA preservation in human skeletal remains. human remains ATR-FTIR spectroscopy preservation state pre-screening method človeški ostanki ATR-FTIR spektroskopija stanje ohranjenosti true false true Angleški jezik Slovenski jezik Članek v reviji 2023-05-17 14:42:05 2023-05-17 14:42:11 2023-05-18 04:00:40 0000-00-00 00:00:00 2023 0 0 Str. 56-64 Vol. 660 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.4 1040-6182 10.1016/j.quaint.2022.09.004 123458819 1-s2.0-S1040618222003044-main.pdf 1-s2.0-S1040618222003044-main.pdf 1 A9915A23393C7556253F8E32BE0ACF96 99f659247091a642f1f96a502e2efefec4d2aa38ededc71725277a025e4f6f24 8b65bf0e-f4b0-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168991 https://www.sciencedirect.com/science/article/pii/S1040618222003044 1 fbe07e5b-f4af-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168990 Filozofska fakulteta Medicinska fakulteta 0 0 0