20.500.12556/RUL-145736 The role of luxS in Campylobacter jejuni beyond intercellular signaling The full role of the luxS gene in the biological processes, such as essential amino acid synthesis, nitrogen and pyruvate metabolism, and flagellar assembly, of Campylobacter jejuni has not been clearly described to date. Therefore, in this study, we used a comprehensive approach at the cellular and molecular levels, including transcriptomics and proteomics, to investigate the key role of the luxS gene and compared C. jejuni 11168ΔluxS (luxS mutant) and C. jejuni NCTC 11168 (wild type) strains. Transcriptomic analysis of the luxS mutant grown under optimal conditions revealed upregulation of luxS mutant metabolic pathways when normalized to wild type, including oxidative phosphorylation, carbon metabolism, citrate cycle, biosynthesis of secondary metabolites, and biosynthesis of various essential amino acids. Interestingly, induction of these metabolic pathways was also confirmed by proteomic analysis, indicating their important role in energy production and the growth of C. jejuni. In addition, genes important for the stress response of C. jejuni, including nutrient starvation and oxidative stress, were upregulated. This was also evident in the better survival of the luxS mutant under starvation conditions than the wild type. At the molecular level, we confirmed that metabolic pathways were upregulated under optimal conditions in the luxS mutant, including those important for the biosynthesis of several essential amino acids. This also modulated the utilization of various carbon and nitrogen sources, as determined by Biolog phenotype microarray analysis. In summary, transcriptomic and proteomic analysis revealed key biological differences in tricarboxylic acid (TCA) cycle, pyruvate, nitrogen, and thiamine metabolism as well as lipopolysaccharide biosynthesis in the luxS mutant. Campylobacter jejuni luxS intercellular signaling transcriptome proteome metabolism stress response patogeni mikroorganizmi Campylobacter jejuni luxS medcelično signaliziranje odziv na stres proteomika true false true Angleški jezik Slovenski jezik Članek v reviji 2023-05-11 10:25:43 2023-05-11 10:25:43 2023-05-12 03:35:55 0000-00-00 00:00:00 2023 0 0 17 str. iss. 2 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26 2165-0497 10.1128/spectrum.02572-22 139309059 RAZ_Ramic_Dina_2023.pdf RAZ_Ramic_Dina_2023.pdf 1 AF53091A1359D3F9C422A2A7EB826BBA 83e2e642dece317d9dd3d9baa1cfd80a95ae89ac90422fe58dd1c27bb6dedd5a e6dc3c3c-efd8-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168738 https://journals.asm.org/doi/10.1128/spectrum.02572-22 1 2eee7877-efd5-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168733 Biotehniška fakulteta 0 0 0