20.500.12556/RUL-145648 Estrogenic potency of endocrine disrupting chemicals and their mixtures detected in environmental waters and wastewaters Endocrine disrupting chemicals such as natural and synthetic steroid hormones and bisphenols are among the most important pollutants in the aquatic environment. We performed an environmental chemical analysis of five Slovenian water samples, two rivers, one groundwater, and the influent and effluent of wastewater treatment plants, with a highly sensitive analysis of twenty-five endocrine-disrupting compounds belonging to the groups of natural hormones, synthetic hormones, and bisphenols. Since these compounds are simultaneously present in the environment, it is important to study their individual effects as well as the effects of mixtures. We investigated in vitro the estrogenic potency of selected natural and synthetic steroid hormones and bisphenols detected in surface, ground and waste water in Slovenia using the OECD-validated transactivation assay on the cell line Hela9903. We predicted their mixture effects using the concentration addition model and compared them with experimentally determined values. Two mixing designs were used: a balanced design in which chemicals were combined in proportion to their individual EC$_{50}$ values, and an unbalanced design with compounds in proportion to their measured concentrations in the environmental samples. The estrogenic effects of the experimental mixtures followed the concentration addition model. Real water samples exhibited weaker estrogenic effects, showing the great heterogeneity of the real water samples. bisphenols concentration addition environmental water estrogenic activity hormones mixtures bisfenoli dodatek koncentracij okoljska voda estrogena aktivnost hormoni mešanice hormonski motilci estrogeni true false true Angleški jezik Slovenski jezik Članek v reviji 2023-05-04 03:30:57 2023-05-04 17:02:09 2023-05-05 03:32:31 0000-00-00 00:00:00 2023 0 0 13 str. art. 138712 Vol. 330 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612.43 0045-6535 10.1016/j.chemosphere.2023.138712 150677507 1-s2.0-S0045653523009797-main.pdf 1-s2.0-S0045653523009797-main.pdf 1 D30F5A1EA6616463D239CEDB990AA876 62f1d7166cc9ad8e27047c98e27ca1570b742338ad7621b07b575eaa74a7fbf4 96118f57-ea48-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168607 https://www.sciencedirect.com/science/article/pii/S0045653523009797 1 1e5c061b-ea1b-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168601 Fakulteta za farmacijo 0 0 0