20.500.12556/RUL-145559 E. coli biofilm formation and its susceptibility towards T4 bacteriophages studied in a continuously operating mixing – tubular bioreactor system A system consisting of a connected mixed and tubular bioreactor was designed to study bacterial biofilm formation and the effect of its exposure to bacteriophages under different experimental conditions. The bacterial biofilm inside silicone tubular bioreactor was formed during the continuous pumping of bacterial cells at a constant physiological state for 2 h and subsequent washing with a buffer for 24 h. Monitoring bacterial and bacteriophage concentration along the tubular bioreactor was performed via a piercing method. The presence of biofilm and planktonic cells was demonstrated by combining the piercing method, measurement of planktonic cell concentration at the tubular bioreactor outlet, and optical microscopy. The planktonic cell formation rate was found to be 8.95 × 10$^{−3}$ h$^{−1}$ and increased approximately four-fold (4×) after biofilm exposure to an LB medium. Exposure of bacterial biofilm to bacteriophages in the LB medium resulted in a rapid decrease of biofilm and planktonic cell concentration, to below the detection limit within < 2 h. When bacteriophages were supplied in the buffer, only a moderate decrease in the concentration of both bacterial cell types was observed. After biofilm washing with buffer to remove unadsorbed bacteriophages, its exposure to the LB medium (without bacteriophages) resulted in a rapid decrease in bacterial concentration: again below the detection limit in < 2 h. microbial biofilm continuous tubular bioreactor bacteriophage interaction bacteriophage T4 bakterijski biofilm kontinuirni cevni bioreaktor bakteriofagna interakcija E. coli bakteriofag T4 true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-21 14:02:31 2023-04-21 14:02:32 2023-04-22 03:36:33 0000-00-00 00:00:00 2022 0 0 Str. 2450-2463 iss. 9 Vol. 15 Sep. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.3:578.347 1751-7915 10.1111/1751-7915.14079 110219011 Microbial_Biotechnology_-_2022_-_Lisac_-_E__coli_biofilm_formation_and_its_susceptibility_towards_T4_bacteriophages_studied.pdf Microbial_Biotechnology_-_2022_-_Lisac_-_E__coli_biofilm_formation_and_its_susceptibility_towards_T4_bacteriophages_studied.pdf 1 783F470EE7677D80A787209AD2AD386D f43cb33f1aad14f7cfa60d7008e175f4015c91c170757e410c89a8e28c683114 96f14e65-e03c-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168469 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.14079 1 38c94f56-e03c-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168468 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0