20.500.12556/RUL-145388 Impact of Bacillus subtilis antibiotic bacilysin and Campylobacter jejuni efflux pumps on pathogen survival in mixed biofilms The foodborne pathogen Campylobacter jejuni is typically found in an agricultural environment; in animals, such as birds, as an intestinal commensal; and also in food products, especially fresh poultry meat. Campylobacter interactions within mixed species biofilms are poorly understood, especially at the microscale. We have recently shown that the beneficial bacterium Bacillus subtilis reduces C. jejuni survival and biofilm formation in coculture by secreting the antibiotic bacillaene. We extend these studies here by providing evidence that besides bacillaene, the antagonistic effect of B. subtilis involves a nonribosomal peptide bacilysin and that the fully functional antagonism depends on the quorum-sensing transcriptional regulator ComA. Using confocal laser scanning microscopy, we also show that secreted antibiotics influence the distribution of C. jejuni and B. subtilis cells in the submerged biofilm and decrease the thickness of the pathogen’s biofilm. Furthermore, we demonstrate that genes encoding structural or regulatory proteins of the efflux apparatus system (cmeF and cmeR), respectively, contribute to the survival of C. jejuni during interaction with B. subtilis PS-216. In conclusion, this study demonstrates a strong potential of B. subtilis PS-216 to reduce C. jejuni biofilm growth, which supports the application of the PS-216 strain to pathogen biofilm control. antibiotics Bacillus subtilis Campylobacter jejuni bacillaene bacilysin biofilm formation efflux pumps secondary metabolites antibiotiki bakterijske interakcije bacilen bacilizin biofilm izlivne črpalke sekundarni metaboliti true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-20 10:42:24 2023-04-20 10:42:31 2023-05-24 12:04:03 0000-00-00 00:00:00 2022 0 0 14 str. iss. 4 Vol. 10 Aug. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24/.26 2165-0497 10.1128/spectrum.02156-22 117948419 spectrum.02156-22.pdf spectrum.02156-22.pdf 1 5FB7C3EF91DDEAAAA8355BD5978BB3C2 6a75d41519043314a9d18855609fe6d59b857c69dc77e3be69f49f84b41083ac 7b04efc7-df65-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168289 https://journals.asm.org/doi/10.1128/spectrum.02156-22 0 44af8e44-df57-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168282 Biotehniška fakulteta 0 0 0