20.500.12556/RUL-145362 Single cell RNA sequencing in autoimmune inflammatory rheumatic diseases current applications, challenges and a step toward precision medicine Single cell RNA sequencing (scRNA-seq) represents a new large scale and high throughput technique allowing analysis of the whole transcriptome at the resolution of an individual cell. It has emerged as an imperative method in life science research, uncovering complex cellular networks and providing indices that will eventually lead to the development of more targeted and personalized therapies. The importance of scRNA-seq has been particularly highlighted through the analysis of complex biological systems, in which cellular heterogeneity is a key aspect, such as the immune system. Autoimmune inflammatory rheumatic diseases represent a group of disorders, associated with a dysregulated immune system and high patient heterogeneity in both pathophysiological and clinical aspects. This complicates the complete understanding of underlying pathological mechanisms, associated with limited therapeutic options available and their long-term inefficiency and even toxicity. There is an unmet need to investigate, in depth, the cellular and molecular mechanisms driving the pathogenesis of rheumatic diseases and drug resistance, identify novel therapeutic targets, as well as make a step forward in using stratified and informed therapeutic decisions, which could now be achieved with the use of single cell approaches. This review summarizes the current use of scRNA-seq in studying different rheumatic diseases, based on recent findings from published in vitro, in vivo, and clinical studies, as well as discusses the potential implementation of scRNA-seq in the development of precision medicine in rheumatology. rheumatoid arthritis systemic lupus erythematosus RNA sequencing single cell systemic sclerosis precision medicine revmatoidni artritis sistemski eritematozni lupus sekvenciranje RNA true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-19 09:59:47 2023-04-19 09:59:52 2023-04-20 03:39:11 0000-00-00 00:00:00 2022 0 0 18 str. Vol. 8, art. 822804 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-002 2296-858X 10.3389/fmed.2021.822804 93835779 fmed-08-822804.pdf fmed-08-822804.pdf 1 0CAF3F8CFE9F5EB30B2CE29FA535874B 7ced90f2344230dcc4a71d431fb61cb15d13af170ed3ced3f025b0d9bb280fb3 513e60b5-de88-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168234 https://www.frontiersin.org/articles/10.3389/fmed.2021.822804/full 1 0045f14e-de88-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168233 Medicinska fakulteta 0 0 0