20.500.12556/RUL-145312 Robustness of diffuse reflectance spectra analysis by inverse adding doubling algorithm Analysing diffuse reflectance spectra to extract properties of biological tissue requires modelling of light transport within the tissue, considering its absorption, scattering, and geometrical properties. Due to the layered skin structure, skin tissue models are often divided into multiple layers with their associated optical properties. Typically, in the analysis, some model parameters defining these properties are fixed to values reported in the literature to speed up the fitting process and improve its performance. In the absence of consensus, various studies use different approaches in fixing the model parameters. This study aims to assess the effect of fixing various model parameters in the skin spectra fitting process on the accuracy and robustness of a GPU-accelerated two-layer inverse adding-doubling (IAD) algorithm. Specifically, the performance of the IAD method is determined for noiseless simulated skin spectra, simulated spectra with different levels of noise applied, and in-vivo measured reflectance spectra from hyperspectral images of human hands recorded before, during, and after the arterial occlusion. Our results suggest that fixing multiple parameters to a priori known values generally improves the robustness and accuracy of the IAD algorithm for simulated spectra. However, for in-vivo measured spectra, these values are unknown in advance and fixing optical parameters to incorrect values significantly deteriorates the overall performance. Therefore, we propose a method to improve the fitting performance by pre-estimating model parameters. Our findings could be considered in all future research involving the analysis of diffuse reflectance spectra to extract optical properties of skin tissue. medical physics biomedical optics medicinska fizika biomedicinska optika true false true Angleški jezik Slovenski jezik “© 2022 Optica Publishing Group under the terms of the Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for noncommercial purposes and appropriate attribution is maintained. All other rights are reserved.” (Datum opombe: 17. 4. 2023) Članek v reviji 2023-04-17 08:38:10 2023-04-17 08:38:11 2023-05-29 10:30:35 0000-00-00 00:00:00 2022 0 0 Str. 921-949 no. 2, art. 443880 Vol. 13 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-073:535 2156-7085 10.1364/BOE.443880 94642435 boe-13-2-921.pdf boe-13-2-921.pdf 1 7CD3C7186BA29F7ED82D65CBEC6AEA52 256eed513f4409809098c9e88e548f18605a8297d28d668aa1639446866aee79 ec824bba-dcea-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168129 https://opg.optica.org/boe/fulltext.cfm?uri=boe-13-2-921&id=468663 0 da9d80aa-dcea-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168128 Fakulteta za matematiko in fiziko 0 0 0