20.500.12556/RUL-145264 Inactivation of the enveloped virus phi6 with hydrodynamic cavitation The COVID-19 pandemic reminded us that we need better contingency plans to prevent the spread of infectious agents and the occurrence of epidemics or pandemics. Although the transmissibility of SARS-CoV-2 in water has not been confirmed, there are studies that have reported on the presence of infectious coronaviruses in water and wastewater samples. Since standard water treatments are not designed to eliminate viruses, it is of utmost importance to explore advanced treatment processes that can improve water treatment and help inactivate viruses when needed. This is the first study to investigate the effects of hydrodynamic cavitation on the inactivation of bacteriophage phi6, an enveloped virus used as a SARS-CoV-2 surrogate in many studies. In two series of experiments with increasing and constant sample temperature, virus reduction of up to 6.3 logs was achieved. Inactivation of phi6 at temperatures of 10 and 20 °C occurs predominantly by the mechanical effect of cavitation and results in a reduction of up to 4.5 logs. At 30 °C, the reduction increases to up to 6 logs, where the temperature-induced increased susceptibility of the viral lipid envelope makes the virus more prone to inactivation. Furthermore, the control experiments without cavitation showed that the increased temperature alone is not sufficient to cause inactivation, but that additional mechanical stress is still required. The RNA degradation results confirmed that virus inactivation was due to the disrupted lipid bilayer and not to RNA damage. Hydrodynamic cavitation, therefore, has the potential to inactivate current and potentially emerging enveloped pathogenic viruses in water at lower, environmentally relevant temperatures. enveloped viruses phi6 SARS-CoV-2 virus inactivation hydrodynamic cavitation water decontamination virusi z ovojnico phi6 SARS-CoV-2 inaktivacija virusov hidrodinamična kavitacija dekontaminacija vode true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-14 11:00:52 2023-04-14 11:00:55 2023-10-24 03:30:28 0000-00-00 00:00:00 2023 0 0 8 str. Vol. 95, art. 106400 May 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 532.528:578 1350-4177 10.1016/j.ultsonch.2023.106400 148619523 Zupanc_..._-_Inactivation_of_the_enveloped_virus_phi6_with_hydrodynamic_cavitation.pdf Zupanc_..._-_Inactivation_of_the_enveloped_virus_phi6_with_hydrodynamic_cavitation.pdf 1 AEA6E7B5FA5EE33BA3575AB08B6E18E2 e078e61aef0ca9b3790f3664c055a7a11563a5f6b481ffe964c7c363d658dc85 a1db6978-daa4-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168014 https://www.sciencedirect.com/science/article/pii/S1350417723001128 1 b67c1551-daa2-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=168010 Fakulteta za strojništvo 0 0 0