20.500.12556/RUL-145243 The relevance of physico-chemical properties and protein corona for evaluation of nanoparticles immunotoxicity—in vitro correlation analysis on THP-1 macrophages Alongside physiochemical properties (PCP), it has been suggested that the protein corona of nanoparticles (NPs) plays a crucial role in the response of immune cells to NPs. However, due to the great variety of NPs, target cells, and exposure protocols, there is still no clear relationship between PCP, protein corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP and the protein corona composition of NPs to the THP-1 macrophage response, focusing on selected toxicological endpoints: cell viability, reactive oxygen species (ROS), and cytokine secretion. We analyzed seven commonly used engineered NPs (SiO$_2$, silver, and TiO$_2$) and magnetic NPs. We show that with the exception of silver NPs, all of the tested TiO$_2$ types and SiO$_2$ exhibited moderate toxicities and a transient inflammatory response that was observed as an increase in ROS, IL-8, and/or IL-1β cytokine secretion. We observed a strong correlation between the size of the NPs in media and IL-1β secretion. The induction of IL-1β secretion was completely blunted in NLR family pyrin domain containing 3 (NLRP3) knockout THP-1 cells, indicating activation of the inflammasome. The correlations analysis also implicated the association of specific NP corona proteins with the induction of cytokine secretion. This study provides new insights toward a better understanding of the relationships between PCP, protein corona, and the inflammatory response of macrophages for different engineered NPs, to which we are exposed on a daily basis. physico-chemical properties nanoparticles nanotoxicology immune response correlation protein corona cytokines inflammasome macrophages TiO$_2$ fizikalno-kemijske lastnosti nanodelci nanotoksikologija imunski odziv korelacija proteinska korona citokini inflamasom makrofagi TiO$_2$ true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-13 14:17:26 2023-04-13 14:17:28 2023-04-14 03:50:00 0000-00-00 00:00:00 2022 0 0 23 str. iss. 11, art. 6197 Vol. 23 Jun. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1422-0067 10.3390/ijms23116197 109968387 ijms-23-06197-v2.pdf ijms-23-06197-v2.pdf 1 50733326B5DC6AFD2CBCABDA7E7096ED f65201a1f7d9ec30d4d7eaaf0757b080ac6aaa21e017d5102f0a7a7c5d1103e9 3fff37b4-d9f5-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167974 https://www.mdpi.com/1422-0067/23/11/6197 1 0261003b-d9f5-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167973 Medicinska fakulteta Fakulteta za elektrotehniko 0 0 0