20.500.12556/RUL-145234 Cobalt ferrite magnetic nanoparticles for tracing mesenchymal stem cells in tissue a preliminary study Therapy with mesenchymal stem cells (MSCs) is promising in many diseases. Evaluation of their efficacy depends on adequate follow-up of MSCs after transplantation. Several studies have shown that MSCs can be labeled and subsequently visualized with magnetic nanoparticles (NPs). We investigated the homing of MSCs labeled with magnetic cobalt ferrite NPs in experimentally induced acute kidney injury in mice. To explore the homing of MSCs after systemic infusion into mice, we developed a pre-infusion strategy for optimal tracing and identification of MSCs with polyacrylic acid-coated cobalt ferrite (CoFe$_2$O$_4$) NPs by light and transmission electron microscopy (TEM) in various organs of mice with cisplatin-induced acute kidney injury and control mice. By correlative microscopy, we detected MSCs labeled with NPs in the lungs, spleen, kidney, and intestine of cisplatin-treated mice and in the lungs and spleen of control mice. Our results confirm that labeling MSCs with metal NPs did not affect the ultrastructure of MSCs and their ability to settle in various organs. This study demonstrates the usefulness of cobalt ferrite NPs in ex vivo visualization of MSCs and offers correlative microscopy as a useful method in routine histopathology laboratories for tracing MSCs in paraffin-embedded tissue. mesenchymal stem cells metal nanoparticles tissue injury markers mezenhimske izvorne celice kovinski nanodelci poškodba tkiva true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-13 12:24:12 2023-04-13 12:24:16 2023-04-14 03:49:58 0000-00-00 00:00:00 2022 0 0 12 str. iss. 15, art. 8738 Vol. 23 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 1422-0067 10.3390/ijms23158738 121139203 ijms-23-08738-v2.pdf ijms-23-08738-v2.pdf 1 90F9B0C7BA23E19B5102C061A3F7843E ed0ff6f5a61dfb2d832aa2a90ffd5b302c9dabd3c14ed52c10486308556254fa 702750de-d9e5-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167958 https://www.mdpi.com/1422-0067/23/15/8738 1 31c3afab-d9e5-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167957 Medicinska fakulteta 0 0 0