20.500.12556/RUL-145180 Design and validation of a custom-made laboratory hyperspectral imaging system for biomedical applications using a broadband LED light source Hyperspectral imaging (HSI) is a promising optical modality that is already being used in numerous applications. Further expansion of the capabilities of HSI depends on the modularity and versatility of the systems, which would, inter alia, incorporate profilometry, fluorescence imaging, and Raman spectroscopy while following a rigorous calibration and verification protocols, thus offering new insights into the studied samples as well as verifiable, quantitative measurement results applicable to the development of quantitative metrics. Considering these objectives, we developed a custom-made laboratory HSI system geared toward biomedical applications. In this report, we describe the design, along with calibration, characterization, and verification protocols needed to establish such systems, with the overall goal of standardization. As an additional novelty, our HSI system uses a custom-built broadband LED-based light source for reflectance imaging, which is particularly important for biomedical applications due to the elimination of sample heating. Three examples illustrating the utility and advantages of the integrated system in biomedical applications are shown. Our attempt presents both the development of a custom-based laboratory HSI system with novel LED light source as well as a framework which may improve technological standards in HSI system design. physics biomedical optics LED light-sources hyperspectral imaging spectroscopy system calibration instrumentation fizika biomedicinska optika LED svetlobni viri hiperspektralno snemanje spektroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-04-12 13:21:34 2023-04-12 13:21:34 2023-04-13 03:31:25 0000-00-00 00:00:00 2022 0 0 23 str. iss. 16, art. 6274 Vol. 22 Aug. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 53 1424-8220 10.3390/s22166274 118725379 sensors-22-06274-v2.pdf sensors-22-06274-v2.pdf 1 66B518A1319007290F7C5DC4517E78A8 552b06d66bc6423f3c8f500c52b5a163f526273dff48e4a68158733644ead397 78bd4c13-d924-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167881 https://www.mdpi.com/1424-8220/22/16/6274 0 31bff58d-d924-11ed-a314-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=167880 Fakulteta za matematiko in fiziko 0 0 0